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Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases
المؤلفون المشاركون
Cheng, Lifeng
Duan, Shengwen
Feng, Xiangyuan
Zheng, Ke
Yang, Qi
Liu, Zhengchu
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-27
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
β -mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation.
This study separated and purified the β -mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a “two-step” method comprising ultrafiltration and gel chromatography.
The purified β -mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg).
The optimum temperature and pH were 65°C and 6.0, respectively.
Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4.5–7.0.
The β -mannanase activity was significantly enhanced in the presence of Mn2+, Cu2+, Zn2+, Ca2+, Mg2+, and Al3+ and strongly inhibited by Ba2+ and Pb2+.
K m and V m a x values for locust bean gum were 7.14 mg/mL and 107.5 μmol/min/mL versus 1.749 mg/mL and 33.45 µmol/min/mL for Konjac glucomannan, respectively.
Therefore, β -mannanase purified by this work shows stability at high temperatures and in weakly acidic or neutral environments.
Based on such data, the β -mannanase will have potential applications as a dietary supplement in treatment of inflammatory processes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cheng, Lifeng& Duan, Shengwen& Feng, Xiangyuan& Zheng, Ke& Yang, Qi& Liu, Zhengchu. 2016. Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098445
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cheng, Lifeng…[et al.]. Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098445
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cheng, Lifeng& Duan, Shengwen& Feng, Xiangyuan& Zheng, Ke& Yang, Qi& Liu, Zhengchu. Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098445
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098445
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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