Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages
المؤلفون المشاركون
Abdalla, Dulcinéia Saes
Saavedra, Nicolás
Salazar, Luis A.
Saavedra, Kathleen
Cuevas, Alejandro
Zambrano, Tomás
Cavalcante, Marcela F.
Dörr, Felipe A.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-28
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Polyphenols from diverse sources have shown anti-inflammatory activity.
In the context of atherosclerosis, macrophages play important roles including matrix metalloproteinases synthesis involved in degradation of matrix extracellular components affecting the atherosclerotic plaque stability.
We prepared a propolis extract and pinocembrin in ethanol solution.
Propolis extract was chemically characterized using LC-MS.
The effect of treatments on gene expression and proteolytic activity was measured in vitro using murine macrophages activated with LPS.
Cellular toxicity associated with both treatments and the vehicle was determined using MTT and apoptosis/necrosis detection assays.
MMP-9 gene expression and proteolytic activity were measured using qPCR and zymography, respectively.
Thirty-two compounds were identified in the propolis extract, including pinocembrin among its major components.
Treatment with either ethanolic extract of propolis or pinocembrin inhibits MMP-9 gene expression in a dose-dependent manner.
Similarly, an inhibitory effect was observed in proteolytic activity.
However, the effect showed by ethanolic extract of propolis was higher than the effect of pinocembrin, suggesting that MMP-9 inhibition results from a joint contribution between the components of the extract.
These data suggest a potential role of polyphenols from Chilean propolis in the control of extracellular matrix degradation in atherosclerotic plaques.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Saavedra, Nicolás& Cuevas, Alejandro& Cavalcante, Marcela F.& Dörr, Felipe A.& Saavedra, Kathleen& Zambrano, Tomás…[et al.]. 2016. Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098503
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Saavedra, Nicolás…[et al.]. Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1098503
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Saavedra, Nicolás& Cuevas, Alejandro& Cavalcante, Marcela F.& Dörr, Felipe A.& Saavedra, Kathleen& Zambrano, Tomás…[et al.]. Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098503
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098503
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر