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Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−Culture+ TB Patients
المؤلفون المشاركون
Liu, Zhiqiang
Qie, Shuang
Li, Lili
Xiu, Bingshui
Yang, Xiqin
Dai, Zhenhua
Zhang, Xuhui
Que, Haiping
Zhao, Ping
Johnson, Heather
Zhang, Heqiu
Feng, Xiaoyan
Duan, Cuimi
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Rapid and accurate diagnosis of pulmonary tuberculosis (PTB) is an unresolved problem worldwide, especially for sputum smear− (S−) cases.
In this study, five antigen genes including Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 were cloned from Mycobacterium tuberculosis (Mtb) RD1 and overexpressed to generate antigen fragments.
These antigens and their combinations were investigated for PTB serodiagnosis.
298 serum samples were collected from active PTB patients, including 117 sputum smear+ (S+) and sputum culture+ (C+) cases, 101 S−/C+ cases, and 80 S−/C− cases.
The serum IgG levels of the five antigens were measured by ELISA.
Based on IgG levels, the sensitivity/specificity of Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 for PTB detection was 81.21%/74.74%, 63.09%/94.78%, 32.21%/87.37%, 62.42%/85.26%, and 83.56%/83.16%, respectively.
Furthermore, the optimal result for PTB diagnosis was achieved by combining antigens Rv3871, Rv3876, and Rv3879.
In addition, the IgG levels of Rv3871, Rv3876, and Rv3879 were found to be higher in S−/C+ PTB patients than in other PTB populations.
More importantly, combination of the three antigens demonstrated superior diagnostic performance for both S−/C+ and S−/C− PTB.
In conclusion, the combination of Rv3871, Rv3876, and Rv3879 induced higher IgG response in sputum S−/C+ PTB patients and represents a promising biomarker combination for diagnosing of PTB.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Zhiqiang& Qie, Shuang& Li, Lili& Xiu, Bingshui& Yang, Xiqin& Dai, Zhenhua…[et al.]. 2016. Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−Culture+ TB Patients. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098799
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Zhiqiang…[et al.]. Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−Culture+ TB Patients. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1098799
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Zhiqiang& Qie, Shuang& Li, Lili& Xiu, Bingshui& Yang, Xiqin& Dai, Zhenhua…[et al.]. Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−Culture+ TB Patients. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098799
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098799
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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