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Dhcr7 Regulates Palatal Shelf Fusion through Regulation of Shh and Bmp2 Expression
المؤلفون المشاركون
Xiao, Wen-lin
Zhang, Dai-zun
Xu, Hong
Zhuang, Cui-zhu
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-15
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The aim of this study was to investigate the effect of the 7-dehydrocholesterol reductase (Dhcr7) gene and identify signaling pathways involved in regulation of embryonic palatogenesis.
The expression of Dhcr7 and its protein product were examined during murine normal embryonic palatogenesis via a reverse transcription polymerase chain reaction (RT-PCR) and Western blot (WB).
RNA interference (RNAi) technology was used to inhibit Dhcr7 expression in a palatal shelf culture in vitro.
The effects of Dhcr7 on palatogenesis and palatal fusion were examined by scanning electron microscopy (SEM).
The expression changes of Dhcr7, Sonic Hedgehog (Shh), and bone morphogenetic protein-2 (Bmp2) were measured by RT-PCR and WB after Dhcr7 gene silencing and the addition of exogenous cholesterol.
The results showed that the palatal shelf failed to complete normal development and fusion when Dhcr7 expression was inhibited.
The inhibitory effect study of RNAi on the development of the palatal shelf supported that cholesterol supplementation did not alter the silencing of Dhcr7.
Shh and Bmp2 expressions were reduced after Dhcr7 gene silencing, and administration of exogenous cholesterol did not affect Dhcr7 expression; however Shh and Bmp2 expressions increased.
We conclude that Dhcr7 plays a role in growth of the palatal shelf and can regulate palatogenesis through alterations in the levels of Shh and Bmp2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xiao, Wen-lin& Zhang, Dai-zun& Xu, Hong& Zhuang, Cui-zhu. 2016. Dhcr7 Regulates Palatal Shelf Fusion through Regulation of Shh and Bmp2 Expression. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098811
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xiao, Wen-lin…[et al.]. Dhcr7 Regulates Palatal Shelf Fusion through Regulation of Shh and Bmp2 Expression. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098811
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xiao, Wen-lin& Zhang, Dai-zun& Xu, Hong& Zhuang, Cui-zhu. Dhcr7 Regulates Palatal Shelf Fusion through Regulation of Shh and Bmp2 Expression. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098811
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098811
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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