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Sequence- and Structure-Based Functional Annotation and Assessment of Metabolic Transporters in Aspergillus oryzae: A Representative Case Study
المؤلفون المشاركون
Vongsangnak, Wanwipa
Raethong, Nachon
Wong-ekkabut, Jirasak
Laoteng, Kobkul
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-04
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Aspergillus oryzae is widely used for the industrial production of enzymes.
In A.
oryzae metabolism, transporters appear to play crucial roles in controlling the flux of molecules for energy generation, nutrients delivery, and waste elimination in the cell.
While the A.
oryzae genome sequence is available, transporter annotation remains limited and thus the connectivity of metabolic networks is incomplete.
In this study, we developed a metabolic annotation strategy to understand the relationship between the sequence, structure, and function for annotation of A.
oryzae metabolic transporters.
Sequence-based analysis with manual curation showed that 58 genes of 12,096 total genes in the A.
oryzae genome encoded metabolic transporters.
Under consensus integrative databases, 55 unambiguous metabolic transporter genes were distributed into channels and pores (7 genes), electrochemical potential-driven transporters (33 genes), and primary active transporters (15 genes).
To reveal the transporter functional role, a combination of homology modeling and molecular dynamics simulation was implemented to assess the relationship between sequence to structure and structure to function.
As in the energy metabolism of A.
oryzae, the H+-ATPase encoded by the AO090005000842 gene was selected as a representative case study of multilevel linkage annotation.
Our developed strategy can be used for enhancing metabolic network reconstruction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Raethong, Nachon& Wong-ekkabut, Jirasak& Laoteng, Kobkul& Vongsangnak, Wanwipa. 2016. Sequence- and Structure-Based Functional Annotation and Assessment of Metabolic Transporters in Aspergillus oryzae: A Representative Case Study. BioMed Research International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1098979
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Raethong, Nachon…[et al.]. Sequence- and Structure-Based Functional Annotation and Assessment of Metabolic Transporters in Aspergillus oryzae: A Representative Case Study. BioMed Research International No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1098979
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Raethong, Nachon& Wong-ekkabut, Jirasak& Laoteng, Kobkul& Vongsangnak, Wanwipa. Sequence- and Structure-Based Functional Annotation and Assessment of Metabolic Transporters in Aspergillus oryzae: A Representative Case Study. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1098979
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098979
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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