Functions of thga1 Gene in Trichoderma harzianum Based on Transcriptome Analysis
المؤلفون المشاركون
Sun, Qing
Jiang, Xiliang
Pang, Li
Wang, Lirong
Li, Mei
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Trichoderma spp.
are important biocontrol filamentous fungi, which are widely used for their adaptability, broad antimicrobial spectrum, and various antagonistic mechanisms.
In our previous studies, we cloned thga1 gene encoding GαI protein from Trichoderma harzianum Th-33.
Its knockout mutant showed that the growth rate, conidial yield, cAMP level, antagonistic action, and hydrophobicity decreased.
Therefore, Illumina RNA-seq technology (RNA-seq) was used to determine transcriptomic differences between the wild-type strain and thga1 mutant.
A total of 888 genes were identified as differentially expressed genes (DEGs), including 427 upregulated and 461 downregulated genes.
All DEGs were assigned to KEGG pathway databases, and 318 genes were annotated in 184 individual pathways.
KEGG analysis revealed that these unigenes were significantly enriched in metabolism and degradation pathways.
GO analysis suggested that the majority of DEGs were associated with catalytic activities and metabolism processes that encode carbohydrate-active enzymes, secondary metabolites, secreted proteins, or transcription factors.
According to the functional annotation of these DEGs by KOG, the most abundant group was “secondary metabolite biosynthesis, transport, and catabolism.” Further studies for functional characterization of candidate genes and pathways reported in this paper are necessary to further define the G protein signaling system in T.
harzianum.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Qing& Jiang, Xiliang& Pang, Li& Wang, Lirong& Li, Mei. 2016. Functions of thga1 Gene in Trichoderma harzianum Based on Transcriptome Analysis. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1099031
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Qing…[et al.]. Functions of thga1 Gene in Trichoderma harzianum Based on Transcriptome Analysis. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1099031
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Qing& Jiang, Xiliang& Pang, Li& Wang, Lirong& Li, Mei. Functions of thga1 Gene in Trichoderma harzianum Based on Transcriptome Analysis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1099031
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1099031
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر