miR-20b Inhibits T Cell Proliferation and Activation via NFAT Signaling Pathway in Thymoma-Associated Myasthenia Gravis
المؤلفون المشاركون
Xin, Yanzhong
Cai, Hongfei
Lu, Tianyu
Zhang, Yan
Yang, Yue
Cui, Youbin
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-19
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Purpose.
We examined the role of miR-20b in development of thymoma-associated myasthenia gravis, especially in T cell proliferation and activation.
Materials and Methods.
Using qRT-PCR, we assessed expression levels of miR-20b and its target genes in cultured cells and patient samples and examined the proliferation of cultured cells, using MTT cell proliferation assays and flow cytometry based cell cycle analysis.
Activation of T cells was determined by both flow cytometry and qRT-PCR of activation-specific marker genes.
Results.
Expression of miR-20b was downregulated in samples of thymoma tissues and serum from patients with thymoma-associated myasthenia gravis.
In addition, T cell proliferation and activation were inhibited by ectopic overexpression of miR-20b, which led to increased T cell proliferation and activation.
NFAT5 and CAMTA1 were identified as targets of miR-20b.
Expression levels of NFAT5 and CAMTA1 were inhibited by miR-20b expression in cultured cells, and the expression levels of miR-20b and NFAT5/CAMTA1 were inversely correlated in patients with thymoma-associated myasthenia gravis.
Conclusion.
miR-20b acts as a tumor suppressor in the development of thymoma and thymoma-associated myasthenia gravis.
The tumor suppressive function of miR-20b in thymoma could be due to its inhibition of NFAT signaling by repression of NFAT5 and CAMTA1 expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xin, Yanzhong& Cai, Hongfei& Lu, Tianyu& Zhang, Yan& Yang, Yue& Cui, Youbin. 2016. miR-20b Inhibits T Cell Proliferation and Activation via NFAT Signaling Pathway in Thymoma-Associated Myasthenia Gravis. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1099330
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xin, Yanzhong…[et al.]. miR-20b Inhibits T Cell Proliferation and Activation via NFAT Signaling Pathway in Thymoma-Associated Myasthenia Gravis. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1099330
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xin, Yanzhong& Cai, Hongfei& Lu, Tianyu& Zhang, Yan& Yang, Yue& Cui, Youbin. miR-20b Inhibits T Cell Proliferation and Activation via NFAT Signaling Pathway in Thymoma-Associated Myasthenia Gravis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1099330
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1099330
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر