Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?
المؤلفون المشاركون
Emamgholipour, Solaleh
Ansari, Mohammad
Hossein-Nezhad, Arash
المصدر
Biochemistry Research International
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-11
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Purpose.
We aimed to investigate the possible effects of melatonin on gene expressions and activities of MnSOD and catalase under conditions of oxidative stress induced by hydrogen peroxide (H2O2) in peripheral blood mononuclear cells (PBMCs).
Materials and Methods.
PBMCs were isolated from healthy subjects and treated as follows: (1) control (only with 0.1% DMSO for 12 h); (2) melatonin (1 mM) for 12 h; (3) H2O2 (250 μM) for 2 h; (4) H2O2 (250 μM) for 2 h following 10 h pretreatment with melatonin (1 mM).
The gene expression was evaluated by real-time PCR.
MnSOD and catalase activities in PBMCs were determined by colorimetric assays.
Results.
Pretreatment of PBMCs with melatonin significantly augmented expression and activity of MnSOD which were diminished by H2O2.
Melatonin treatment of PBMCs caused a significant upregulation of catalase by almost 2-fold in comparison with untreated cells.
However, activity and expression of catalase increased by 1.5-fold in PBMCs under H2O2-induced oxidative stress compared with untreated cell.
Moreover, pretreatment of PBMCs with melatonin resulted in a significant 1.8-fold increase in catalase expression compared to PBMCs treated only with H2O2.
Conclusion.
It seems that melatonin could prevent from undesirable impacts of H2O2-induced oxidative stress on MnSOD downregulation.
Moreover, melatonin could promote inductive effect of H2O2 on catalase mRNA expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Emamgholipour, Solaleh& Hossein-Nezhad, Arash& Ansari, Mohammad. 2016. Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?. Biochemistry Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099486
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Emamgholipour, Solaleh…[et al.]. Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?. Biochemistry Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099486
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Emamgholipour, Solaleh& Hossein-Nezhad, Arash& Ansari, Mohammad. Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?. Biochemistry Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099486
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1099486
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر