High Virulence and Antifungal Resistance in Clinical Strains of Candida albicans
المؤلفون المشاركون
Monroy-Pérez, Eric
Paniagua-Contreras, Gloria Luz
Rodríguez-Purata, Pamela
Vaca-Paniagua, Felipe
Vázquez-Villaseñor, Marco
Díaz-Velásquez, Clara
Uribe-García, Alina
Vaca, Sergio
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-12
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Antifungal resistance and virulence properties of Candida albicans are a growing health problem worldwide.
To study the expression of virulence and azole resistance genes in 39 clinical strains of C.
albicans, we used a model of infection of human vaginal epithelial cells with C.
albicans strains isolated from Mexican women with vulvovaginal candidiasis (VVC).
The strains were identified by PCR amplification of the ITS1 and ITS2 regions of rRNA.
The detection and expression of virulence genes and azole resistance genes MDR1 and CDR1 were performed using PCR and RT-PCR, respectively.
All strains were sensitive to nystatin and 38 (97.4%) and 37 (94.9%) were resistant to ketoconazole and fluconazole, respectively.
ALS1, SAP4–SAP6, LIP1, LIP2, LIP4, LIP6, LIP7, LIP9, LIP10, and PLB1-PLB2 were present in all strains; SAP1 was identified in 37 (94.8%) isolates, HWP1 in 35 (89.7%), ALS3 in 14 (35.8%), and CDR1 in 26 (66.6%).
In nearly all of the strains, ALS1, HWP1, SAP4–SAP6, LIP1–LIP10, PLB1, and PLB2 were expressed, whereas CDR1 was expressed in 20 (51.3%) and ALS3 in 14 (35.8%).
In our in vitro model of infection with C.
albicans, the clinical strains showed different expression profiles of virulence genes in association with the azole resistance gene CDR1.
The results indicate that the strains that infect Mexican patients suffering from VVC are highly virulent and virtually all are insensitive to azoles.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Monroy-Pérez, Eric& Paniagua-Contreras, Gloria Luz& Rodríguez-Purata, Pamela& Vaca-Paniagua, Felipe& Vázquez-Villaseñor, Marco& Díaz-Velásquez, Clara…[et al.]. 2016. High Virulence and Antifungal Resistance in Clinical Strains of Candida albicans. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1100002
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Monroy-Pérez, Eric…[et al.]. High Virulence and Antifungal Resistance in Clinical Strains of Candida albicans. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1100002
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Monroy-Pérez, Eric& Paniagua-Contreras, Gloria Luz& Rodríguez-Purata, Pamela& Vaca-Paniagua, Felipe& Vázquez-Villaseñor, Marco& Díaz-Velásquez, Clara…[et al.]. High Virulence and Antifungal Resistance in Clinical Strains of Candida albicans. Canadian Journal of Infectious Diseases and Medical Microbiology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1100002
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1100002
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر