High-Throughput Analysis of the T Cell Receptor Beta Chain Repertoire in PBMCs from Chronic Hepatitis B Patients with HBeAg Seroconversion

المؤلفون المشاركون

Tong, Yigang
An, Xiaoping
Mi, Zhiqiang
Qu, Yachao
Huang, Yong
Liu, Di
Huang, Yinuo
Zhang, Zhiyi
Lu, Jun

المصدر

Canadian Journal of Infectious Diseases and Medical Microbiology

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-10-13

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الأحياء

الملخص EN

T lymphocytes are the most important immune cells that affect both the development and treatment of hepatitis B.

We used high-throughput sequencing to determine the diversity in the V and J regions of the TCRβ chain in 4 chronic hepatitis B patients before and after HBeAg seroconversion.

Here, we demonstrate that the 4 patients expressed Vβ12-4 at the highest frequencies of 10.6%, 9.2%, 17.5%, and 7.5%, and Vβ28 was the second most common, with frequencies of 7.8%, 6.7%, 5.3%, and 10.9%, respectively.

No significant changes were observed following seroconversion.

With regard to the Jβ gene, Jβ2-1 was the most commonly expressed in the 4 patients at frequencies of 5.8%, 6.5%, 11.3%, and 7.3%, respectively.

Analysis of the V-J region genes revealed several differences, including significant increases in the expression levels of V7-2-01-J2-1, V12-4-J1-1, and V28-1-J1-5 and a decrease in that of V19-01-J2-3.

These results illustrate the presence of biased TCRVβ and Jβ gene expression in the chronic hepatitis B patients.

TRBVβ12-4, Vβ28, Jβ2-1, V7-2-01-J2-1, V12-4-J1-1, and V28-1-J1-5 may be associated with the development and treatment of CHB.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Qu, Yachao& Huang, Yong& Liu, Di& Huang, Yinuo& Zhang, Zhiyi& Mi, Zhiqiang…[et al.]. 2016. High-Throughput Analysis of the T Cell Receptor Beta Chain Repertoire in PBMCs from Chronic Hepatitis B Patients with HBeAg Seroconversion. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1100020

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Qu, Yachao…[et al.]. High-Throughput Analysis of the T Cell Receptor Beta Chain Repertoire in PBMCs from Chronic Hepatitis B Patients with HBeAg Seroconversion. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1100020

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Qu, Yachao& Huang, Yong& Liu, Di& Huang, Yinuo& Zhang, Zhiyi& Mi, Zhiqiang…[et al.]. High-Throughput Analysis of the T Cell Receptor Beta Chain Repertoire in PBMCs from Chronic Hepatitis B Patients with HBeAg Seroconversion. Canadian Journal of Infectious Diseases and Medical Microbiology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1100020

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1100020