Biochemical and Molecular Analysis of Staphylococcus aureus Clinical Isolates from Hospitalized Patients
المؤلفون المشاركون
Karmakar, Amit
Dua, Parimal
Ghosh, Chandradipa
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-04-24
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Staphylococcus aureus is opportunistic human as well as animal pathogen that causes a variety of diseases.
A total of 100 Staphylococcus aureus isolates were obtained from clinical samples derived from hospitalized patients.
The presumptive Staphylococcus aureus clinical isolates were identified phenotypically by different biochemical tests.
Molecular identification was done by PCR using species specific 16S rRNA primer pairs and finally 100 isolates were found to be positive as Staphylococcus aureus.
Screened isolates were further analyzed by several microbiological diagnostics tests including gelatin hydrolysis, protease, and lipase tests.
It was found that 78%, 81%, and 51% isolates were positive for gelatin hydrolysis, protease, and lipase activities, respectively.
Antibiogram analysis of isolated Staphylococcus aureus strains with respect to different antimicrobial agents revealed resistance pattern ranging from 57 to 96%.
Our study also shows 70% strains to be MRSA, 54.3% as VRSA, and 54.3% as both MRSA and VRSA.
All the identified isolates were subjected to detection of mecA, nuc, and hlb genes and 70%, 84%, and 40% were found to harbour mecA, nuc, and hlb genes, respectively.
The current investigation is highly important and informative for the high level multidrug resistant Staphylococcus aureus infections inclusive also of methicillin and vancomycin.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Karmakar, Amit& Dua, Parimal& Ghosh, Chandradipa. 2016. Biochemical and Molecular Analysis of Staphylococcus aureus Clinical Isolates from Hospitalized Patients. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1100024
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Karmakar, Amit…[et al.]. Biochemical and Molecular Analysis of Staphylococcus aureus Clinical Isolates from Hospitalized Patients. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1100024
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Karmakar, Amit& Dua, Parimal& Ghosh, Chandradipa. Biochemical and Molecular Analysis of Staphylococcus aureus Clinical Isolates from Hospitalized Patients. Canadian Journal of Infectious Diseases and Medical Microbiology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1100024
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1100024
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر