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Validation of an LC-MSMS Method for Urinary Lactulose and Mannitol Quantification: Results in Patients with Irritable Bowel Syndrome
المؤلفون المشاركون
Primiano, Aniello
Gervasoni, Jacopo
Giorgio, Valentina
Russo, Consuelo
Scaldaferri, Franco
Urbani, Andrea
Zuppi, Cecilia
Persichilli, Silvia
Schiattarella, Arcangelo
Tesori, Valentina
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-14
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Aim.
Lactulose/mannitol ratio is used to assess intestinal barrier function.
Aim of this work was to develop a robust and rapid method for the analysis of lactulose and mannitol in urine by liquid chromatography coupled to tandem mass spectrometry.
Lactulose/mannitol ratio has been measured in pediatric patients suffering from irritable bowel syndrome.
Methods.
Calibration curves and raffinose, used as internal standard, were prepared in water : acetonitrile 20 : 80.
Fifty μL of urine sample was added to 450 μL of internal standard solution.
The chromatographic separation was performed using a Luna NH2 column operating at a flow rate of 200 μL/min and eluted with a linear gradient from 20% to 80% water in acetonitrile.
Total run time is 9 minutes.
The mass spectrometry operates in electrospray negative mode.
Method was fully validated according to European Medicine Agency guidelines.
Results and Conclusions.
Linearity ranged from 10 to 1000 mg/L for mannitol and 2.5 to 1000 mg/L for lactulose.
Imprecision in intra- and interassay was lower than 15% for both analytes.
Accuracy was higher than 85%.
Lactulose/mannitol ratio in pediatric patients is significantly higher than that measured in controls.
The presented method, rapid and sensitive, is suitable in a clinical laboratory.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gervasoni, Jacopo& Schiattarella, Arcangelo& Giorgio, Valentina& Primiano, Aniello& Russo, Consuelo& Tesori, Valentina…[et al.]. 2016. Validation of an LC-MSMS Method for Urinary Lactulose and Mannitol Quantification: Results in Patients with Irritable Bowel Syndrome. Disease Markers،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1103726
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gervasoni, Jacopo…[et al.]. Validation of an LC-MSMS Method for Urinary Lactulose and Mannitol Quantification: Results in Patients with Irritable Bowel Syndrome. Disease Markers No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1103726
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gervasoni, Jacopo& Schiattarella, Arcangelo& Giorgio, Valentina& Primiano, Aniello& Russo, Consuelo& Tesori, Valentina…[et al.]. Validation of an LC-MSMS Method for Urinary Lactulose and Mannitol Quantification: Results in Patients with Irritable Bowel Syndrome. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1103726
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1103726
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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