Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells
المؤلفون المشاركون
Yang, Wen-Tao
Li, Gen-Hua
Li, Zheng-You
Feng, Song
Liu, Xue-Qin
Han, Guang-Kui
Zhang, Hao
Qin, Xian-Yun
Zhang, Ran
Nie, Quan-Min
Jin, Feng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-09
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
This study is to explore the effect of corilagin on the proliferation and NF-κB signaling pathway in U251 glioblastoma cells and U251 glioblastoma stem-like cells.
Methods.
CD133 positive U251 glioblastoma cells were separated by immunomagnetic beads to isolate glioblastoma stem-like cells.
U251 cells and stem-like cells were intervened by different corilagin concentrations (0, 25, 50, and 100 μg/mL) for 48 h, respectively.
Cell morphology, cell counting kit-8 assay, flow cytometry, dual luciferase reporter assay, and a western blot were used to detect and analyze the cell proliferation and cell cycle and investigate the expression of IKBα protein in cytoplasm and NF-κB/p65 in nucleus.
Results.
Corilagin inhibited the cell proliferation of U251 cells and their stem-like cells and the inhibition role was stronger in U251 stem-like cells ( P < 0.05 ).
The cell cycle was arrested at G2/M phase in the U251 cells following corilagin intervention; the proportion of cells in G2/M phase increased as the concentration of corilagin increased ( P < 0.05 ).
The U251 stem-like cells were arrested at the S phase following treatment with corilagin; the proportion of cells in the S phase increased as the concentration of corilagin increased ( P < 0.05 ).
The ratio of dual luciferase activities of U251 stem-like cells was lower than that of U251 cells in the same corilagin concentration.
With increasing concentrations of corilagin, the IKBα expression in cytoplasm of U251 cells and U251 stem-like cells was increased, but the p65 expression in nucleus of U251 cells and U251 stem-like cells was decreased ( P < 0.05 ).
Conclusion.
Corilagin can inhibit the proliferation of glioblastoma cells and glioblastoma stem-like cells; the inhibition on glioblastoma stem-like cell proliferation is stronger than glioblastoma cells.
This different result indicates that the effect of corilagin on U251 cells and U251 stem-like cells may have close relationships with mechanism of cell cycle and NF-κB signaling pathway; however, the real antitumor mechanism of corilagin is not yet clear and requires further study.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Wen-Tao& Li, Gen-Hua& Li, Zheng-You& Feng, Song& Liu, Xue-Qin& Han, Guang-Kui…[et al.]. 2016. Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1103902
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Wen-Tao…[et al.]. Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1103902
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Wen-Tao& Li, Gen-Hua& Li, Zheng-You& Feng, Song& Liu, Xue-Qin& Han, Guang-Kui…[et al.]. Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1103902
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1103902
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر