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The Extract of Fructus Psoraleae Promotes Viability and Cartilaginous Formation of Rat Chondrocytes In Vitro
المؤلفون المشاركون
Pan, Xin
Xu, Kang
Qiu, Xuefeng
Zhao, Wen
Wang, Dong
Yang, Li
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-11-23
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
This study aimed to investigate the extract components of FP on rat chondrocyte function and cartilaginous formation in vitro.
Petroleum ether extract (P-e) of FP extract components was selected to treat Sprague-Dawley rat chondrocytes.
Cell viability was tested with different concentrations (0.1, 1, 10, and 100 μg/mL) of P-e treatment.
Concentrations of 0.1 and 1 μg/mL P-e conditioned culture mediums were used for treating chondrocytes in experiments.
Cell proliferation was measured via DNA incorporation assay.
Type II collagen, aggrecan, and Sox-9 genes expression levels were measured with RT-PCR.
Additionally, cartilaginous formation was analyzed with type II collagen immunofluorescence, H&E, and alcian blue staining.
Concentrations of 0.1 and 1 μg/mL P-e showed low cytotoxicity and demonstrated stimulatory effects on chondrocyte proliferation in early stages.
Following 6 days of P-e culture, aggrecan and Sox-9 gene expression levels of the 1 μg/mL P-e group were upregulated by 1.82- (p<0.05) and 2.06-fold (p<0.05), respectively, versus controls.
Moreover, 1 μg/mL P-e significantly stimulated cell aggregation and type II collagen deposits after 1 week of treatment.
Noteworthy, tight cartilaginous structures formed in the 10-day 1 μg/mL P-e conditioned culture.
These findings suggest that P-e has the potential to treat cartilage degeneration induced by chondrocyte failure.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pan, Xin& Xu, Kang& Qiu, Xuefeng& Zhao, Wen& Wang, Dong& Yang, Li. 2016. The Extract of Fructus Psoraleae Promotes Viability and Cartilaginous Formation of Rat Chondrocytes In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1103963
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pan, Xin…[et al.]. The Extract of Fructus Psoraleae Promotes Viability and Cartilaginous Formation of Rat Chondrocytes In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1103963
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pan, Xin& Xu, Kang& Qiu, Xuefeng& Zhao, Wen& Wang, Dong& Yang, Li. The Extract of Fructus Psoraleae Promotes Viability and Cartilaginous Formation of Rat Chondrocytes In Vitro. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1103963
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1103963
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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