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iTRAQ-Based Proteomic Analysis of Ginsenoside F2 on Human Gastric Carcinoma Cells SGC7901
المؤلفون المشاركون
Mao, Qian
Zhang, Pin-Hu
Yang, Jie
Xu, Jin-Di
Kong, Ming
Shen, Hong
Zhu, He
Bai, Min
Zhou, Li
Li, Guang-Fu
Wang, Qiang
Li, Song-Lin
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-21، 21ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-18
دولة النشر
مصر
عدد الصفحات
21
التخصصات الرئيسية
الملخص EN
Ginsenoside F2 (F2), a protopanaxdiol type of saponin, was reported to inhibit human gastric cancer cells SGC7901.
To better understand the molecular mechanisms of F2, an iTRAQ-based proteomics approach was applied to define protein expression profiles in SGC7901 cells in response to lower dose (20 μM) and shorter duration (12 hour) of F2 treatment, compared with previous study.
205 proteins were screened in terms of the change in their expression level which met our predefined criteria.
Further bioinformatics and experiments demonstrated that F2 treatment downregulated PRR5 and RPS15 and upregulated RPL26, which are implicated in ribosomal protein-p53 signaling pathway.
F2 also inhibited CISD2, Bcl-xl, and NLRX1, which are associated with autophagic pathway.
Furthermore, it was demonstrated that F2 treatment increased Atg5, Atg7, Atg10, and PUMA, the critical downstream effectors of ribosomal protein-p53 signaling pathway, and Beclin-1, UVRAG, and AMBRA-1, the important molecules in Bcl-xl/Beclin-1 pathway.
The 6 differentially abundant proteins, PRR5, CISD2, Bcl-xl, NLRX1, RPS15, and RPL26, were confirmed by western blot.
Taken together, ribosomal protein-p53 signaling pathway and Bcl-xl/Beclin-1 pathway might be the most significantly regulated biological process by F2 treatment in SGC7901 cells, which provided valuable insights into the deep understanding of the molecular mechanisms of F2 for gastric cancer treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mao, Qian& Zhang, Pin-Hu& Yang, Jie& Xu, Jin-Di& Kong, Ming& Shen, Hong…[et al.]. 2016. iTRAQ-Based Proteomic Analysis of Ginsenoside F2 on Human Gastric Carcinoma Cells SGC7901. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-21.
https://search.emarefa.net/detail/BIM-1104006
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mao, Qian…[et al.]. iTRAQ-Based Proteomic Analysis of Ginsenoside F2 on Human Gastric Carcinoma Cells SGC7901. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-21.
https://search.emarefa.net/detail/BIM-1104006
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mao, Qian& Zhang, Pin-Hu& Yang, Jie& Xu, Jin-Di& Kong, Ming& Shen, Hong…[et al.]. iTRAQ-Based Proteomic Analysis of Ginsenoside F2 on Human Gastric Carcinoma Cells SGC7901. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-21.
https://search.emarefa.net/detail/BIM-1104006
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104006
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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