Antibacterial and Anti-Inflammatory Activities of Physalis Alkekengi var. franchetii and Its Main Constituents

الملخص EN

This study was designed to determine whether the 50% EtOH fraction from AB-8 macroporous resin fractionation of a 70% EtOH extract of P.

Alkekengi (50-EFP) has antibacterial and/or anti-inflammatory activity both in vivo and in vitro and to investigate the mechanism of 50-EFP anti-inflammatory activity.

Additionally, this study sought to define the chemical composition of 50-EFP.

Results indicated that 50-EFP showed significant antibacterial activity in vitro and efficacy in vivo.

Moreover, 50-EFP significantly reduced nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF- α), interleukin 1 (IL-1), and interleukin 6 (IL-6) production in lipopolysaccharide- (LPS-) stimulated THP-1 cells.

Nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) (examined at the protein level) in THP-1 cells were suppressed by 50-EFP, which inhibited nuclear translocation of p65.

Consistent with this anti-inflammatory activity in vitro, 50-EFP reduced inflammation in both animal models.

Finally, seventeen compounds (8 physalins and 9 flavones) were isolated as major components of 50-EFP.

Our data demonstrate that 50-EFP has antibacterial and anti-inflammatory activities both in vitro and in vivo.

The anti-inflammatory effect appears to occur, at least in part, through the inhibition of nuclear translocation of p65.

Moreover, physalins and flavones are probably the active components in 50-EFP that exert antibacterial and anti-inflammatory activities.