Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury
المؤلفون المشاركون
Tan, Lingjing
Zhang, Xiang
Mei, Zhigang
Wang, Jinfeng
Li, Xiaoli
Huang, Weifeng
Yang, Songbai
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-21
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Fermented Chinese formula Shuan-Tong-Ling (STL), composed of fourteen medicinal herbs, was an experiential formula by Dr.
Zhigang Mei for treating vascular encephalopathy, but the underlying mechanisms remained unknown.
In this study, we aimed to investigate the protective effects of fermented STL on hydrogen peroxide- (H2O2-) induced injury in rat brain microvascular endothelial cells (BMECs) and the possible mechanisms.
Cultured BMECs were treated with H2O2, STL, or nicotinamide (NAM, a SIRT1 inhibitor).
Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was employed to detect cell proliferation and senescence-associated beta-galactosidase (SA-β-gal) was used to examine cell senescence.
Cell nuclei were observed by 4′,6-diamidino-2-phenylindole.
Additionally, changes in reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione (GSH) levels were measured.
Expression of SIRT1, p21, and PGC-1α was determined by western blot.
Cell proliferation significantly increased with STL treatment in a dose-dependent manner.
H2O2 treatment could intensify cell senescence and nuclei splitting or pyknosis.
With STL treatment, the reduced ROS level was accompanied by increased SOD and GSH activity.
Further assays showed upregulation of SIRT1 and PGC-1α and downregulation of p21 after STL treatment.
The results revealed that STL could protect BMECs against oxidative stress injury at least partially through the SIRT1 pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tan, Lingjing& Zhang, Xiang& Mei, Zhigang& Wang, Jinfeng& Li, Xiaoli& Huang, Weifeng…[et al.]. 2016. Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104195
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tan, Lingjing…[et al.]. Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1104195
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tan, Lingjing& Zhang, Xiang& Mei, Zhigang& Wang, Jinfeng& Li, Xiaoli& Huang, Weifeng…[et al.]. Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104195
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104195
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر