Naoxintong Protects Primary Neurons from Oxygen-Glucose DeprivationReoxygenation Induced Injury through PI3K-Akt Signaling Pathway
المؤلفون المشاركون
Zhu, Jin-qiang
Gao, Xiu-Mei
Ma, Yan
Zhao, Pei
Yan, Chen
Li, Lin
Zhang, Han
Zhang, Meng
Fan, Xiang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-02-02
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Naoxintong capsule (NXT), developed from Buyang Huanwu Decoction, has shown the neuroprotective effects in cerebrovascular diseases, but the neuroprotection mechanisms of NXT on ischemia/reperfusion injured neurons have not yet been well known.
In this study, we established the oxygen-glucose deprivation/reoxygenation (OGD/R) induced neurons injury model and treat the neurons with cerebrospinal fluid containing NXT (BNC) to investigate the effects of NXT on OGD/R induced neurons injury and potential mechanisms.
BNC improved neuron viability and decreased apoptotic rate induced by OGD/R.
BNC attenuated OGD/R induced cytosolic and mitochondrial Ca2+ overload, ROS generation, intracellular NO levels and nNOS mRNA increase, and cytochrome-c release when compared with OGD/R group.
BNC significantly inhibited both mPTP opening and Δ Ψ m depolarization.
BNC increased Bcl-2 expression and decreased Bax expression, upregulated the Bcl-2/Bax ratio, downregulated caspase-3 mRNA and caspase-9 mRNA expression, and decreased cleaved caspase-3 expression and caspase-3 activity.
BNC increased phosphorylation of Akt following OGD/R, while LY294002 attenuated BNC induced increase of phosphorylated Akt expression.
Our study demonstrated that NXT protected primary neurons from OGD/R induced injury by inhibiting calcium overload and ROS generation, protecting mitochondria, and inhibiting mitochondrial apoptotic pathway which was mediated partially by PI3K-Akt signaling pathway activation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ma, Yan& Zhao, Pei& Zhu, Jin-qiang& Yan, Chen& Li, Lin& Zhang, Han…[et al.]. 2016. Naoxintong Protects Primary Neurons from Oxygen-Glucose DeprivationReoxygenation Induced Injury through PI3K-Akt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1104230
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ma, Yan…[et al.]. Naoxintong Protects Primary Neurons from Oxygen-Glucose DeprivationReoxygenation Induced Injury through PI3K-Akt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1104230
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ma, Yan& Zhao, Pei& Zhu, Jin-qiang& Yan, Chen& Li, Lin& Zhang, Han…[et al.]. Naoxintong Protects Primary Neurons from Oxygen-Glucose DeprivationReoxygenation Induced Injury through PI3K-Akt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1104230
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104230
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر