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A Bioactivity-Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum
المؤلفون المشاركون
Chang, Yanxu
Gao, Xiu-Mei
Li, Jin
Ge, Ai-hua
Jiang, Yan
Teye Azietaku, John
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-26
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Traditional Chinese medicine (TCM) has been used for the treatment of many complex diseases.
However, the bioactive components are always undefined.
In this study, a bioactivity-based method was developed and validated to screen lipase inhibitors and evaluate the effects of processing on the lipase inhibitory activity of TCM by ultrahigh performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry and fraction collector (UHPLC/Q-TOF-MS-FC).
The results showed that both Polygonum multiflorum and processed P.
multiflorum extracts had inhibitory effect against lipase with IC50 values of 38.84 μg/mL and 190.6 μg/mL, respectively.
Stilbenes, phenolic acid, flavonoids, and anthraquinones were considered to be the potential lipase inhibitors.
Eleven potential lipase inhibitors were simultaneously determined by UHPLC.
Principal component analysis (PCA) was employed in exploring the effects of processing time on lipase inhibitory activity of P.
multiflorum.
Compared with conventional methods, a bioactivity-based method could quantitatively analyze lipase inhibitory activity of individual constituent and provide the total lipase inhibitory activity of the samples.
The results demonstrated that the activity integrated UHPLC/Q-TOF-MS-FC method was an effective and powerful tool for screening and identifying lipase inhibitors from traditional Chinese medicines.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chang, Yanxu& Ge, Ai-hua& Jiang, Yan& Teye Azietaku, John& Li, Jin& Gao, Xiu-Mei. 2016. A Bioactivity-Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104249
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chang, Yanxu…[et al.]. A Bioactivity-Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1104249
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chang, Yanxu& Ge, Ai-hua& Jiang, Yan& Teye Azietaku, John& Li, Jin& Gao, Xiu-Mei. A Bioactivity-Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104249
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104249
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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