Essential Oil of Ocimum basilicum L. and (−)‎-Linalool Blocks the Excitability of Rat Sciatic Nerve

الملخص EN

The racemate linalool and its levogyrus enantiomer [(−)-LIN] are present in many essential oils and possess several pharmacological activities, such as antinociceptive and anti-inflammatory.

In this work, the effects of essential oil obtained from the cultivation of the Ocimum basilicum L.

(EOOb) derived from Germplasm Bank rich in (−)-LIN content in the excitability of peripheral nervous system were studied.

We used rat sciatic nerve to investigate the EOOb and (−)-LIN effects on neuron excitability and the extracellular recording technique was used to register the compound action potential (CAP).

EOOb and (−)-LIN blocked the CAP in a concentration-dependent way and these effects were reversible after washout.

EOOb blocked positive amplitude of 1st and 2nd CAP components with IC50 of 0.38±0.2 and 0.17±0.0 mg/mL, respectively.

For (−)-LIN, these values were 0.23±0.0 and 0.13±0.0 mg/mL.

Both components reduced the conduction velocity of CAP and the 2nd component seems to be more affected than the 1st component.

In conclusion EOOb and (−)-LIN inhibited the excitability of peripheral nervous system in a similar way and potency, revealing that the effects of EOOb on excitability are due to the presence of (−)-LIN in the essential oil.