Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013)
المؤلفون المشاركون
Hong-ying, Zhang
Meng-kai, Qiao
Xuan, Wang
Xie, Guo-xiang
Jin, Hei-ying
Li-min, Shi
Min, He
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-31
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Aim.
To gain insight into the molecular diversity of sapovirus in outpatients with acute gastroenteritis in Nanjing, China.
Methods.
The specimens from outpatients clinically diagnosed as acute gastroenteritis were detected by real-time PCR; RT-PCR was then performed to amplify part of VP1 sequences.
The PCR products were cloned into pGEM-T Easy vector and bidirectionally sequenced.
All sequences were edited and analyzed.
A phylogenetic tree was drawn with the MEGA 5.0 software.
Results.
Between 2011 and 2013, 16 sapovirus positive cases were confirmed by real-time PCR.
The infected cases increased from two in 2011 and six in 2012 to eight in 2013.
The majority was children and the elderly (15, 93.75%) and single infections (15, 93.75%).
Of the 16 real-time positive specimens, 14 specimens had PCR products and the analysis data of the 14 nucleic sequences showed that there was one GI genogroup with four genotypes, two GI.2 in 2011, three GI.2, and one GI.1 in 2012 and one GI.2, three GI.1, two GI.3, and two GI.5 in 2013.
Conclusion.
Our data confirmed continuous existing of GI genogroup and GI.2 genotype from 2011 to 2013 in Nanjing and the successive appearance of different genotypes from outpatients with gastroenteritis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hong-ying, Zhang& Meng-kai, Qiao& Xuan, Wang& Min, He& Li-min, Shi& Xie, Guo-xiang…[et al.]. 2016. Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013). Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104681
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hong-ying, Zhang…[et al.]. Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013). Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1104681
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hong-ying, Zhang& Meng-kai, Qiao& Xuan, Wang& Min, He& Li-min, Shi& Xie, Guo-xiang…[et al.]. Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013). Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104681
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104681
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر