Detection of Bacterial Wilt Pathogen and Isolation of Its Bacteriophage from Banana in Lumajang Area, Indonesia
المؤلفون المشاركون
Addy, Hardian Susilo
Azizi, Norita Fatatik
Mihardjo, Paniman Ashna
المصدر
International Journal of Agronomy
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-22
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Bacterial wilt disease on banana is an important disease in Lumajang District and causes severe yield loss.
Utilizing bacteriophage as natural enemy of pathogenic bacteria has been widely known as one of the control strategies.
This research was aimed at determining the causing agent of bacterial wilt on banana isolated from Lumajang area, to obtain wide-host range bacteriophages against bacterial wilt pathogen and to know the basic characteristic of bacteriophages, particularly its nucleic acid type.
Causative agent of bacterial wilt was isolated from symptomatic banana trees from seven districts in Lumajang area on determinative CPG plates followed by rapid detection by PCR technique using specific pair-primer.
Bacteriophages were also isolated from soil of infected banana crop in Sukodono District.
Morphological observation showed that all bacterial isolates have similar characteristic as common bacterial wilt pathogen, Ralstonia solanacearum.
In addition, detection of FliC region in all isolates confirmed that all isolates were R.
solanacearum according to the presence of 400 bp of FliC DNA fragment.
Moreover, two bacteriophages were obtained from this experiment ( ϕ RSSKD1 and ϕ RSSKD2), which were able to infect all nine R.
solanacearum isolates.
Nucleic acid analysis showed that the nucleic acid of bacteriophages was DNA (deoxyribonucleic acid).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Addy, Hardian Susilo& Azizi, Norita Fatatik& Mihardjo, Paniman Ashna. 2016. Detection of Bacterial Wilt Pathogen and Isolation of Its Bacteriophage from Banana in Lumajang Area, Indonesia. International Journal of Agronomy،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104904
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Addy, Hardian Susilo…[et al.]. Detection of Bacterial Wilt Pathogen and Isolation of Its Bacteriophage from Banana in Lumajang Area, Indonesia. International Journal of Agronomy No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1104904
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Addy, Hardian Susilo& Azizi, Norita Fatatik& Mihardjo, Paniman Ashna. Detection of Bacterial Wilt Pathogen and Isolation of Its Bacteriophage from Banana in Lumajang Area, Indonesia. International Journal of Agronomy. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104904
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104904
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر