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Measurement of Circulating 1,25-Dihydroxyvitamin D: Comparison of an Automated Method with a Liquid Chromatography Tandem Mass Spectrometry Method
المؤلفون المشاركون
Ernst, Jana B.
Becker, Tobias
Dreier, Jens
Knabbe, Cornelius
Kuhn, Joachim
Gummert, Jan F.
Zittermann, Armin
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-04-05
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
The clinical relevance of circulating 1,25-dihydroxyvitamin D (1,25(OH)2D) is probably underappreciated, but variations in the measurement of this difficult analyte between different methods limit comparison of results.
Methods.
In 129 clinical samples, we compared a new automated assay with a commercially available liquid chromatography tandem mass spectrometry (LC-MS/MS) kit.
Results.
Median (interquartile range) 1,25(OH)2D concentrations with the automated assay and the LC-MS/MS method were 26.6 pg/mL (18.5–39.0 pg/mL) and 23.6 pg/mL (16.1–31.3 pg/mL), respectively ( P = 0.001 ).
Using the method-specific cut-offs for deficient 1,25(OH)2D levels (<20 pg/mL for the automated assay and <17 pg/mL for the LC-MS/MS method), the percentage of patients classified as 1,25(OH)2D deficient was 28.7% and 27.1%, respectively.
However, concordance between the two methods for deficient levels was only 62% and the concordance correlation coefficient was poor (0.534).
The regression equation resulted in an intercept of −1.99 (95% CI: −7.33–1.31) and a slope of 1.27 (95% CI: 1.04–1.52) for the automated assay.
The mean bias with respect to the mean of the two methods was −3.8 (1.96 SD: −28.3–20.8) pg/mL for the LC-MS/MS method minus the automated assay.
Conclusions.
The two methods show only modest correlation and further standardization is required to improve reliability and comparability of 1,25(OH)2D test procedures.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zittermann, Armin& Ernst, Jana B.& Becker, Tobias& Dreier, Jens& Knabbe, Cornelius& Gummert, Jan F.…[et al.]. 2016. Measurement of Circulating 1,25-Dihydroxyvitamin D: Comparison of an Automated Method with a Liquid Chromatography Tandem Mass Spectrometry Method. International Journal of Analytical Chemistry،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104957
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zittermann, Armin…[et al.]. Measurement of Circulating 1,25-Dihydroxyvitamin D: Comparison of an Automated Method with a Liquid Chromatography Tandem Mass Spectrometry Method. International Journal of Analytical Chemistry No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1104957
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zittermann, Armin& Ernst, Jana B.& Becker, Tobias& Dreier, Jens& Knabbe, Cornelius& Gummert, Jan F.…[et al.]. Measurement of Circulating 1,25-Dihydroxyvitamin D: Comparison of an Automated Method with a Liquid Chromatography Tandem Mass Spectrometry Method. International Journal of Analytical Chemistry. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104957
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104957
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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