![](/images/graphics-bg.png)
Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil
المؤلفون المشاركون
Olofsson, Madelen A.
Bylund, Dan
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-02-09
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
This method employs liquid chromatography-tandem mass spectrometry to rapidly quantify chitin-derived glucosamine for estimating fungal biomass.
Analyte retention was achieved using hydrophilic interaction liquid chromatography, with a zwitter-ionic stationary phase (ZIC-HILIC), and isocratic elution using 60% 5 mM ammonium formate buffer (pH 3.0) and 40% ACN.
Inclusion of muramic acid and its chromatographic separation from glucosamine enabled calculation of the bacterial contribution to the latter.
Galactosamine, an isobaric isomer to glucosamine, found in significant amounts in soil samples, was also investigated.
The two isomers form the same precursor and product ions and could not be chromatographically separated using this rapid method.
Instead, glucosamine and galactosamine were distinguished mathematically, using the linear relationships describing the differences in product ion intensities for the two analytes.
The m/z transitions of 180 → 72 and 180 → 84 were applied for the detection of glucosamine and galactosamine and that of 252 → 126 for muramic acid.
Limits of detection were in the nanomolar range for all included analytes.
The total analysis time was 6 min, providing a high sample throughput method.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Olofsson, Madelen A.& Bylund, Dan. 2016. Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil. International Journal of Analytical Chemistry،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104959
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Olofsson, Madelen A.& Bylund, Dan. Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil. International Journal of Analytical Chemistry No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1104959
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Olofsson, Madelen A.& Bylund, Dan. Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil. International Journal of Analytical Chemistry. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104959
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104959
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)