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Oxidative Nanopatterning of Titanium Surface Influences mRNA and MicroRNA Expression in Human Alveolar Bone Osteoblastic Cells
المؤلفون المشاركون
Passos, Geraldo Aleixo
Wimmers Ferreira, Maidy Rehder
Rodrigo Fernandes, Roger
Freire Assis, Amanda
Dernowsek, Janaína A.
Variola, Fabio
Fittipaldi Bombonato-Prado, Karina
المصدر
International Journal of Biomaterials
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-04-21
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Titanium implants have been extensively used in orthopedic and dental applications.
It is well known that micro- and nanoscale surface features of biomaterials affect cellular events that control implant-host tissue interactions.
To improve our understanding of how multiscale surface features affect cell behavior, we used microarrays to evaluate the transcriptional profile of osteoblastic cells from human alveolar bone cultured on engineered titanium surfaces, exhibiting the following topographies: nanotexture (N), nano+submicrotexture (NS), and rough microtexture (MR), obtained by modulating experimental parameters (temperature and solution composition) of a simple yet efficient chemical treatment with a H2SO4/H2O2 solution.
Biochemical assays showed that cell culture proliferation augmented after 10 days, and cell viability increased gradually over 14 days.
Among the treated surfaces, we observed an increase of alkaline phosphatase activity as a function of the surface texture, with higher activity shown by cells adhering onto nanotextured surfaces.
Nevertheless, the rough microtexture group showed higher amounts of calcium than nanotextured group.
Microarray data showed differential expression of 716 mRNAs and 32 microRNAs with functions associated with osteogenesis.
Results suggest that oxidative nanopatterning of titanium surfaces induces changes in the metabolism of osteoblastic cells and contribute to the explanation of the mechanisms that control cell responses to micro- and nanoengineered surfaces.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wimmers Ferreira, Maidy Rehder& Rodrigo Fernandes, Roger& Freire Assis, Amanda& Dernowsek, Janaína A.& Passos, Geraldo Aleixo& Variola, Fabio…[et al.]. 2016. Oxidative Nanopatterning of Titanium Surface Influences mRNA and MicroRNA Expression in Human Alveolar Bone Osteoblastic Cells. International Journal of Biomaterials،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1105474
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wimmers Ferreira, Maidy Rehder…[et al.]. Oxidative Nanopatterning of Titanium Surface Influences mRNA and MicroRNA Expression in Human Alveolar Bone Osteoblastic Cells. International Journal of Biomaterials No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1105474
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wimmers Ferreira, Maidy Rehder& Rodrigo Fernandes, Roger& Freire Assis, Amanda& Dernowsek, Janaína A.& Passos, Geraldo Aleixo& Variola, Fabio…[et al.]. Oxidative Nanopatterning of Titanium Surface Influences mRNA and MicroRNA Expression in Human Alveolar Bone Osteoblastic Cells. International Journal of Biomaterials. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1105474
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1105474
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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