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IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania
المؤلفون المشاركون
Lyimo, Beatus
Buza, Joram
Subbiah, Murugan
Temba, Sylivester
Kipasika, Honest
Miller, W. A.
Call, Douglas R.
المصدر
International Journal of Microbiology
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-24
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
The aim of this study was to identify the replicon types of plasmids, conjugation efficiencies, and the complement of antibiotic resistance genes for a panel of multidrug resistant E.
coli isolates from surface waters in northern Tanzania.
Standard membrane filtration was used to isolate and uidA PCR was used to confirm the identity of strains as E.
coli.
Antibiotic susceptibility was determined by breakpoint assay and plasmid conjugation was determined by filter-mating experiments.
PCR and sequencing were used to identify resistance genes and PCR-based replicon typing was used to determine plasmid types.
Filter mating experiments indicated conjugation efficiencies ranged from 10−1 to 10−7.
Over 80% of the donor cells successfully passed their resistance traits and eleven different replicon types were detected (IncI1, FIC, P, FIIA, A/C, FIB, FIA, H12, K/B B/O, and N).
IncF plasmids were most commonly detected (49% of isolates), followed by types IncI1 and IncA/C.
Detection of these public health-relevant conjugative plasmids and antibiotic resistant traits in Tanzanian water suggests the possible pollution of these water sources from human, livestock, and wild animal wastes and also shows the potential of these water sources in the maintenance and transmission of these resistance traits between environments, animals, and people.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lyimo, Beatus& Buza, Joram& Subbiah, Murugan& Temba, Sylivester& Kipasika, Honest& Miller, W. A.…[et al.]. 2016. IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106260
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lyimo, Beatus…[et al.]. IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania. International Journal of Microbiology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1106260
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lyimo, Beatus& Buza, Joram& Subbiah, Murugan& Temba, Sylivester& Kipasika, Honest& Miller, W. A.…[et al.]. IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106260
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1106260
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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