Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity
المؤلفون المشاركون
Yamada, Shizuka
Capati, Mark Luigi Fabian
Nakazono, Ayako
Yamamoto, Kohei
Yanagiguchi, Kajiro
Sugimoto, Kouji
Hayashi, Yoshihiko
المصدر
International Journal of Polymer Science
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-20
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Tilapia type I atelocollagen (TAC) is a strong candidate for clinical application as its biological scaffold due to a high degeneration temperature and biologically safe properties.
The aim of this study was to confirm the biological effects of TAC in vitro on osteoblastic cells, simulating its clinical application.
The proliferation and differentiation of typical preosteoblasts, MC3T3-E1 cells, were investigated using a microarray analysis, staining assay for mineralization, and real-time PCR analysis of the expression of mineralization-related genes.
The mRNA expression of 10 genes involved in proliferation and differentiation increased after 3-day culture on an TAC gel, with an average balanced score ratio exceeding 1.5 compared to the control.
After two weeks of culture, all three experimental groups showed stronger alkaline phosphatase staining than after one week.
The genes expression of alkaline phosphatase, osteocalcin, and bone sialoprotein increased under the experimental conditions.
The gene expression of osteopontin did not increase, and no statistical differences were noted among the three experimental groups.
The present and previous findings suggest that TAC is not only a suitable alternative to collagen products originating from mammals but also a novel biomaterial with cell differentiation ability for regenerative medicine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Capati, Mark Luigi Fabian& Nakazono, Ayako& Yamamoto, Kohei& Sugimoto, Kouji& Yanagiguchi, Kajiro& Yamada, Shizuka…[et al.]. 2016. Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity. International Journal of Polymer Science،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106813
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Capati, Mark Luigi Fabian…[et al.]. Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity. International Journal of Polymer Science No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1106813
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Capati, Mark Luigi Fabian& Nakazono, Ayako& Yamamoto, Kohei& Sugimoto, Kouji& Yanagiguchi, Kajiro& Yamada, Shizuka…[et al.]. Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity. International Journal of Polymer Science. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106813
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1106813
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر