Using MALDI-TOF Mass Spectrometry to Identify Drug Resistant Staphylococcal Isolates from Nonhospital Environments in Brunei Darussalam
المؤلفون المشاركون
Chong, Ko S.
Shazali, Siti A.
Xu, Zhen
Cutler, Ronald R.
Idris, Adi
المصدر
Interdisciplinary Perspectives on Infectious Diseases
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-04-03
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Drug resistant bacteria have been a growing threat to the community and hospitals due to the misuse of antibiotics by humans, industrialization, and lack of novel antimicrobials currently available.
Little is known about the prevalence of drug resistant bacteria in nonhealthcare environments in Brunei Darussalam and about how antibiotic resistant genes are transferred within these environments.
Human contact points from different types of environments in Brunei Darussalam, varying from urban to jungle settings, were swabbed and cultured onto selective media to isolate staphylococci bacteria before performing antimicrobial susceptibility testing on the isolates.
The identity of the isolates was determined using MALDI-TOF mass spectrometry (MS).
Staphylococci isolates resistant to oxacillin were further tested for their minimum inhibitory concentration (MIC).
PCR analysis of the mecA gene, a gene that confers resistance to oxacillin, is done to determine the level of resistance to oxacillin.
Ten different staphylococcal species were identified by MALDI-TOF-MS analysis.
Out of the 36 staphylococci isolates, 24 were resistant to multiple antibiotics including two isolates which were oxacillin resistant.
Some staphylococci isolates had similar antibiotic resistance profiles to other staphylococci isolates of different species in the same location.
This work provides the first-ever evidence of drug resistant staphylococci in the nonhospital environment in Brunei Darussalam.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chong, Ko S.& Shazali, Siti A.& Xu, Zhen& Cutler, Ronald R.& Idris, Adi. 2016. Using MALDI-TOF Mass Spectrometry to Identify Drug Resistant Staphylococcal Isolates from Nonhospital Environments in Brunei Darussalam. Interdisciplinary Perspectives on Infectious Diseases،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1107155
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chong, Ko S.…[et al.]. Using MALDI-TOF Mass Spectrometry to Identify Drug Resistant Staphylococcal Isolates from Nonhospital Environments in Brunei Darussalam. Interdisciplinary Perspectives on Infectious Diseases No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1107155
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chong, Ko S.& Shazali, Siti A.& Xu, Zhen& Cutler, Ronald R.& Idris, Adi. Using MALDI-TOF Mass Spectrometry to Identify Drug Resistant Staphylococcal Isolates from Nonhospital Environments in Brunei Darussalam. Interdisciplinary Perspectives on Infectious Diseases. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1107155
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1107155
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر