Intravascular Residence Time Determination for the Cyanide Antidote Dimethyl Trisulfide in Rat by Using Liquid-Liquid Extraction Coupled with High Performance Liquid Chromatography
المؤلفون المشاركون
De Silva, Deepthika
Lee, Steven
Duke, Anna
Angalakurthi, Siva
Chou, Ching-En
Ebrahimpour, Afshin
Thompson, David E.
Petrikovics, Ilona
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-07
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
These studies represent the first report on the intravascular residence time determinations for the cyanide antidote dimethyl trisulfide (DMTS) in a rat model by using high performance liquid chromatography coupled with ultraviolet absorption spectroscopy (HPLC-UV).
The newly developed sample preparation included liquid-liquid extraction by cyclohexanone.
The calibration curves showed a linear response for DMTS concentrations between 0.010 and 0.30 mg/mL with R2 = 0.9994.
The limit of detection for DMTS via this extraction method was 0.010 mg/mL, and the limit of quantitation was 0.034 mg/mL.
Thus this calibration curve provided a tool for determining DMTS in the range between 0.04 and 0.30 mg/mL.
Rats were given 20 mg/kg DMTS dose (in 15% Polysorbate 80) intravenously, and blood samples were taken 15, 60, 90, 120, and 240 min after DMTS injections.
The data points were plotted as DMTS concentration in RBCs versus time, and the intravascular residence time was determined graphically.
The results indicated a half-life of 36 min in a rat model, suggesting that the circulation time is long enough to provide a reasonable time interval for cyanide antagonism.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
De Silva, Deepthika& Lee, Steven& Duke, Anna& Angalakurthi, Siva& Chou, Ching-En& Ebrahimpour, Afshin…[et al.]. 2016. Intravascular Residence Time Determination for the Cyanide Antidote Dimethyl Trisulfide in Rat by Using Liquid-Liquid Extraction Coupled with High Performance Liquid Chromatography. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1107347
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
De Silva, Deepthika…[et al.]. Intravascular Residence Time Determination for the Cyanide Antidote Dimethyl Trisulfide in Rat by Using Liquid-Liquid Extraction Coupled with High Performance Liquid Chromatography. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1107347
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
De Silva, Deepthika& Lee, Steven& Duke, Anna& Angalakurthi, Siva& Chou, Ching-En& Ebrahimpour, Afshin…[et al.]. Intravascular Residence Time Determination for the Cyanide Antidote Dimethyl Trisulfide in Rat by Using Liquid-Liquid Extraction Coupled with High Performance Liquid Chromatography. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1107347
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1107347
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر