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Analyses of Total Alkaloid Extract of Corydalis yanhusuo by Comprehensive RP × RP Liquid Chromatography with pH Difference
المؤلفون المشاركون
Wei, Xiaodong
Shen, Hongling
Wang, Lijun
Meng, Qingyan
Liu, Wenjie
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A comprehensive two-dimensional (2D) reverse phase (RP) liquid chromatography (LC) method is developed for alkaloid analysis.
This offline comprehensive 2D method is developed using different pH values.
With a pH value of 10.5, most alkaloids appear in the form of neutral molecules possessing high retention factors based on their polarity, while the alkaloid polarity order is changed when the pH value decreased to 3.0.
The performance of pH modulated 2D LC is demonstrated with 8 alkaloid standards which resulted in orthogonal separation.
The developed method is then applied to total alkaloid separation in Corydalis yanhusuo.
The first-dimension separation is carried out using methanol and water containing 1.0% ammonium hydroxide and a strong base-resistant RP column, which afforded a peak capacity of 94.
The second-dimension analysis is carried out with a surface positive charge column providing a peak capacity of 205 using a mobile phase consisting of acetonitrile and water with 0.15% formic acid.
2D analyses of total alkaloid extract from C.
yanhusuo afford a total peak capacity of 9090.
Sixteen compounds were tentatively identified based on their ultraviolet spectrum and MS/MS analyses.
The proposed method provides an alternative approach to achieve high peak capacity for analysis of alkaloid extract.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wei, Xiaodong& Shen, Hongling& Wang, Lijun& Meng, Qingyan& Liu, Wenjie. 2016. Analyses of Total Alkaloid Extract of Corydalis yanhusuo by Comprehensive RP × RP Liquid Chromatography with pH Difference. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107399
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wei, Xiaodong…[et al.]. Analyses of Total Alkaloid Extract of Corydalis yanhusuo by Comprehensive RP × RP Liquid Chromatography with pH Difference. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1107399
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wei, Xiaodong& Shen, Hongling& Wang, Lijun& Meng, Qingyan& Liu, Wenjie. Analyses of Total Alkaloid Extract of Corydalis yanhusuo by Comprehensive RP × RP Liquid Chromatography with pH Difference. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107399
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1107399
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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