![](/images/graphics-bg.png)
Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay
المؤلفون المشاركون
Fietto, Juliana Lopes Rangel
Calil, Felipe Antunes
Lima, Juliana Maria
de Oliveira, Arthur Henrique Cavalcante
Mariotini-Moura, Christiane
Cardoso, Carmen Lucia
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-14
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The use of IMERs (Immobilized Enzyme Reactors) as a stationary phase coupled to high performance chromatographic systems is an interesting approach in the screening of new ligands.
In addition, IMERs offer many advantages over techniques that employ enzymes in solution.
The enzyme nucleoside triphosphate diphosphohydrolase (NTPDase-1) from Trypanosoma cruzi acts as a pathogen infection facilitator, so it is a good target in the search for inhibitors.
In this paper, immobilization of NTPDase-1 afforded ICERs (Immobilized Capillary Enzyme Reactors).
A liquid chromatography method was developed and validated to monitor the ICER activity.
The conditions for the application of these bioreactors were investigated, and excellent results were obtained.
The enzyme was successfully immobilized, as attested by the catalytic activity detected in the TcNTPDase-1-ICER chromatographic system.
Kinetic studies on the substrate ATP gave KM of 0.317 ± 0.044 mmol·L−1, which still presented high affinity compared to in solution.
Besides that, the ICER was stable for 32 days, enough time to investigate samples of possible inhibitors, including especially the compound Suramin, that inhibited 51% the enzyme activity at 100 µmol·L−1, which is in accordance with the data for the enzyme in solution.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Calil, Felipe Antunes& Lima, Juliana Maria& de Oliveira, Arthur Henrique Cavalcante& Mariotini-Moura, Christiane& Fietto, Juliana Lopes Rangel& Cardoso, Carmen Lucia. 2016. Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1107402
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Calil, Felipe Antunes…[et al.]. Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1107402
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Calil, Felipe Antunes& Lima, Juliana Maria& de Oliveira, Arthur Henrique Cavalcante& Mariotini-Moura, Christiane& Fietto, Juliana Lopes Rangel& Cardoso, Carmen Lucia. Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1107402
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1107402
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)