Extraction of Saponin from Camellia oleifera Abel Cake by a Combination Method of Alkali Solution and Acid Isolation

الملخص EN

Saponin 15%~20% content in the seed cake of Camellia oleifera Abel, from which Camellia oil is squeezed, is a natural nonionic surface active agent and is extensively applied to emulsification, humectation, foaming, medicine, pesticide, and so on.

In this paper, the extraction process of saponin was researched through a combining method of alkali solution and acid isolation.

A quantitative method for saponin was established by ultraviolet spectrophotometer.

The influence of extraction factors was investigated by a single-factor test and a response surface methodology.

The results indicated that the optimal extraction conditions of saponin were extraction temperature 68°C, alkali solution pH 9.1, acid isolation pH 4.1, and liquid-solid ratio 15.9 : 1.

The extraction rate of saponin was 76.12% at the optimal extraction conditions.