ALDH2 Inhibition Potentiates High Glucose Stress-Induced Injury in Cultured Cardiomyocytes
المؤلفون المشاركون
Thandavarayan, Rajarajan A.
Pan, Guodong
Palaniyandi, Suresh Selvaraj
Deshpande, Mandar
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-11-02
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Aldehyde dehydrogenase (ALDH) gene superfamily consists of 19 isozymes.
They are present in various organs and involved in metabolizing aldehydes that are biologically generated.
For instance, ALDH2, a cardiac mitochondrial ALDH isozyme, is known to detoxify 4-hydroxy-2-nonenal, a reactive aldehyde produced upon lipid peroxidation in diabetic conditions.
We hypothesized that inhibition of ALDH leads to the accumulation of unmetabolized 4HNE and consequently exacerbates injury in cells subjected to high glucose stress.
H9C2 cardiomyocyte cell lines were pretreated with 10 μM disulfiram (DSF), an inhibitor of ALDH2 or vehicle (DMSO) for 2 hours, and then subjected to high glucose stress {33 mM D-glucose (HG) or 33 mM D-mannitol as an osmotic control (Ctrl)} for 24 hrs.
The decrease in ALDH2 activity with DSF pretreatment was higher in HG group when compared to Ctrl group.
Increased 4HNE adduct formation with DSF pretreatment was higher in HG group compared to Ctrl group.
Pretreatment with DSF leads to potentiated HG-induced cell death in cultured H9C2 cardiomyocytes by lowering mitochondrial membrane potential.
Our results indicate that ALDH2 activity is important in preventing high glucose induced cellular dysfunction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pan, Guodong& Deshpande, Mandar& Thandavarayan, Rajarajan A.& Palaniyandi, Suresh Selvaraj. 2016. ALDH2 Inhibition Potentiates High Glucose Stress-Induced Injury in Cultured Cardiomyocytes. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107959
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pan, Guodong…[et al.]. ALDH2 Inhibition Potentiates High Glucose Stress-Induced Injury in Cultured Cardiomyocytes. Journal of Diabetes Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1107959
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pan, Guodong& Deshpande, Mandar& Thandavarayan, Rajarajan A.& Palaniyandi, Suresh Selvaraj. ALDH2 Inhibition Potentiates High Glucose Stress-Induced Injury in Cultured Cardiomyocytes. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107959
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1107959
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر