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Metformin Protects H9C2 Cardiomyocytes from High-Glucose and HypoxiaReoxygenation Injury via Inhibition of Reactive Oxygen Species Generation and Inflammatory Responses: Role of AMPK and JNK
المؤلفون المشاركون
Hu, Mingyan
Ye, Ping
Liao, Hua
Chen, Manhua
Yang, Feiyan
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-16
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Metformin is a first-line drug for the management of type 2 diabetes.
Recent studies suggested cardioprotective effects of metformin against ischemia/reperfusion injury.
However, it remains elusive whether metformin provides direct protection against hypoxia/reoxygenation (H/R) injury in cardiomyocytes under normal or hyperglycemic conditions.
This study in H9C2 rat cardiomyoblasts was designed to determine cell viability under H/R and high-glucose (HG, 33 mM) conditions and the effects of cotreatment with various concentrations of metformin (0, 1, 5, and 10 mM).
We further elucidated molecular mechanisms underlying metformin-induced cytoprotection, especially the possible involvement of AMP-activated protein kinase (AMPK) and Jun NH(2)-terminal kinase (JNK).
Results indicated that 5 mM metformin improved cell viability, mitochondrial integrity, and respiratory chain activity under HG and/or H/R ( P < 0.05 ).
The beneficial effects were associated with reduced levels of reactive oxygen species generation and proinflammatory cytokines (TNF- α , IL-1 α , and IL-6) ( P < 0.05 ).
Metformin enhanced phosphorylation level of AMPK and suppressed HG + H/R induced JNK activation.
Inhibitor of AMPK (compound C) or activator of JNK (anisomycin) abolished the cytoprotective effects of metformin.
In conclusion, our study demonstrated for the first time that metformin possessed direct cytoprotective effects against HG and H/R injury in cardiac cells via signaling mechanisms involving activation of AMPK and concomitant inhibition of JNK.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hu, Mingyan& Ye, Ping& Liao, Hua& Chen, Manhua& Yang, Feiyan. 2016. Metformin Protects H9C2 Cardiomyocytes from High-Glucose and HypoxiaReoxygenation Injury via Inhibition of Reactive Oxygen Species Generation and Inflammatory Responses: Role of AMPK and JNK. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108042
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hu, Mingyan…[et al.]. Metformin Protects H9C2 Cardiomyocytes from High-Glucose and HypoxiaReoxygenation Injury via Inhibition of Reactive Oxygen Species Generation and Inflammatory Responses: Role of AMPK and JNK. Journal of Diabetes Research No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1108042
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hu, Mingyan& Ye, Ping& Liao, Hua& Chen, Manhua& Yang, Feiyan. Metformin Protects H9C2 Cardiomyocytes from High-Glucose and HypoxiaReoxygenation Injury via Inhibition of Reactive Oxygen Species Generation and Inflammatory Responses: Role of AMPK and JNK. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108042
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108042
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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