Nanoparticle Delivered Human Biliverdin Reductase-Based Peptide Increases Glucose Uptake by Activating IRKAktGSK3 Axis: The Peptide Is Effective in the Cell and Wild-Type and Diabetic ObOb Mice
المؤلفون المشاركون
Gibbs, Peter E. M.
Miralem, Tihomir
Lerner-Marmarosh, Nicole
Maines, Mahin D.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-17
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Insulin’s stimulation of glucose uptake by binding to the IRK extracellular domain is compromised in diabetes.
We have recently described an unprecedented approach to stimulating glucose uptake.
KYCCSRK (P2) peptide, corresponding to the C-terminal segment of hBVR, was effective in binding to and inducing conformational change in the IRK intracellular kinase domain.
Although myristoylated P2, made of L-amino acids, was effective in cell culture, its use for animal studies was unsuitable.
We developed a peptidase-resistant formulation of the peptide that was efficient in both mice and cell culture systems.
The peptide was constructed of D-amino acids, in reverse order, and blocked at both termini.
Delivery of the encapsulated peptide to HepG2 and HSKM cells was confirmed by its prolonged effect on stimulation of glucose uptake (>6 h).
The peptide improved glucose clearance in both wild-type and Ob/Ob mice; it lowered blood glucose levels and suppressed glucose-stimulated insulin secretion.
IRK activity was stimulated in the liver of treated mice and in cultured cells.
The peptide potentiated function of IRK’s downstream effector, Akt-GSK3-(α,β) axis.
Thus, P2-based approach can be used for improving glucose uptake by cells.
Also, it allows for screening peptides in vitro and in animal models for treatment of diabetes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gibbs, Peter E. M.& Miralem, Tihomir& Lerner-Marmarosh, Nicole& Maines, Mahin D.. 2016. Nanoparticle Delivered Human Biliverdin Reductase-Based Peptide Increases Glucose Uptake by Activating IRKAktGSK3 Axis: The Peptide Is Effective in the Cell and Wild-Type and Diabetic ObOb Mice. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1108128
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gibbs, Peter E. M.…[et al.]. Nanoparticle Delivered Human Biliverdin Reductase-Based Peptide Increases Glucose Uptake by Activating IRKAktGSK3 Axis: The Peptide Is Effective in the Cell and Wild-Type and Diabetic ObOb Mice. Journal of Diabetes Research No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1108128
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gibbs, Peter E. M.& Miralem, Tihomir& Lerner-Marmarosh, Nicole& Maines, Mahin D.. Nanoparticle Delivered Human Biliverdin Reductase-Based Peptide Increases Glucose Uptake by Activating IRKAktGSK3 Axis: The Peptide Is Effective in the Cell and Wild-Type and Diabetic ObOb Mice. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1108128
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108128
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر