Coenzyme Q10 Attenuates High Glucose-Induced Endothelial Progenitor Cell Dysfunction through AMP-Activated Protein Kinase Pathways

الملخص EN

Coenzyme Q10 (CoQ10), an antiapoptosis enzyme, is stored in the mitochondria of cells.

We investigated whether CoQ10 can attenuate high glucose-induced endothelial progenitor cell (EPC) apoptosis and clarified its mechanism.

EPCs were incubated with normal glucose (5 mM) or high glucose (25 mM) enviroment for 3 days, followed by treatment with CoQ10 (10 μM) for 24 hr.

Cell proliferation, nitric oxide (NO) production, and JC-1 assay were examined.

The specific signal pathways of AMP-activated protein kinase (AMPK), eNOS/Akt, and heme oxygenase-1 (HO-1) were also assessed.

High glucose reduced EPC functional activities, including proliferation and migration.

Additionally, Akt/eNOS activity and NO production were downregulated in high glucose-stimulated EPCs.

Administration of CoQ10 ameliorated high glucose-induced EPC apoptosis, including downregulation of caspase 3, upregulation of Bcl-2, and increase in mitochondrial membrane potential.

Furthermore, treatment with CoQ10 reduced reactive oxygen species, enhanced eNOS/Akt activity, and increased HO-1 expression in high glucose-treated EPCs.

These effects were negated by administration of AMPK inhibitor.

Transplantation of CoQ10-treated EPCs under high glucose conditions into ischemic hindlimbs improved blood flow recovery.

CoQ10 reduced high glucose-induced EPC apoptosis and dysfunction through upregulation of eNOS, HO-1 through the AMPK pathway.

Our findings provide a potential treatment strategy targeting dysfunctional EPC in diabetic patients.