Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes
المؤلفون المشاركون
Bayrasheva, Valentina K.
Dobronravov, Vladimir A.
Dmitriev, Yuri V.
Chefu, Svetlana G.
Pchelin, Ivan Yu.
Ivanova, Alexandra N.
Alexeyeva, Nina P.
Shatalov, Ivan S.
Bairamov, Alekber
Babenko, A. Y.
Elena, Grineva
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-20
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
Type 2 diabetes (DM2) could be reproduced in rats with alimentary obesity by using low doses of streptozotocin (LD-STZ) as well as STZ in high doses with preliminary nicotinamide (NA) administration.
However, STZ could induce tubulotoxicity.
Aim.
To develop rat model of DN in NA-STZ-induced DM2 and compare it with LD-STZ-model in order to choose the most relevant approach for reproducing renal glomerular and tubular morphofunctional diabetic changes.
Starting at 3 weeks after uninephrectomy, adult male Wistar rats were fed five-week high-fat diet and then received intraperitoneally either LD-STZ (40 mg/kg) or NA (230 mg/kg) followed by STZ (65 mg/kg).
Control uninephrectomized vehicle-injected rats received normal chow.
At weeks 10, 20, and 30 (the end of the study), metabolic parameters, creatinine clearance, albuminuria, and urinary tubular injury markers (NGAL, KIM-1) were evaluated as well as renal ultrastructural and light microscopic changes at weeks 20 and 30.
NA-STZ-group showed higher reproducibility and stability of metabolic parameters.
By week 10, in NA-STZ-group NGAL level was significantly lower compared to LD-STZ-group.
By week 30, diabetic groups showed early features of DN.
However, morphofunctional changes in NA-STZ-group appeared to be more pronounced than those in STZ-group despite lower levels of KIM-1 and NGAL.
We proposed a new rat model of DM2 with DN characterized by stable metabolic disorders, typical renal lesions, and lower levels of tubular injury markers as compared to LD-STZ-induced diabetes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bayrasheva, Valentina K.& Babenko, A. Y.& Dobronravov, Vladimir A.& Dmitriev, Yuri V.& Chefu, Svetlana G.& Pchelin, Ivan Yu.…[et al.]. 2016. Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1108278
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bayrasheva, Valentina K.…[et al.]. Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes. Journal of Diabetes Research No. 2016 (2016), pp.1-18.
https://search.emarefa.net/detail/BIM-1108278
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bayrasheva, Valentina K.& Babenko, A. Y.& Dobronravov, Vladimir A.& Dmitriev, Yuri V.& Chefu, Svetlana G.& Pchelin, Ivan Yu.…[et al.]. Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1108278
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108278
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر