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Structural and Functional Characterization of Recombinant Interleukin-10 from Indian Major Carp Labeo rohita
المؤلفون المشاركون
Dixit, Aparna
Karan, Sweta
Dash, Pujarini
Kaushik, Himani
Sahoo, Pramoda K.
Garg, Lalit C.
المصدر
Journal of Immunology Research
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-01
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Interleukin-10, an important regulator of both the innate and adaptive immune systems, is a multifunctional major cytokine.
Though it is one of the major cytokines, IL-10 from the Indian major carp, Labeo rohita, has not yet been characterized.
In the present study, we report large scale production and purification of biologically active recombinant IL-10 of L.
rohita (rLrIL-10) using a heterologous expression system and its biophysical and functional characterization.
High yield (~70 mg/L) of soluble rLrIL-10 was obtained at shake flask level.
The rLrIL-10 was found to exist as a dimer.
Far-UV CD spectroscopy showed presence of predominantly alpha helices.
The tertiary structure of the purified rLrIL-10 was verified by fluorescence spectroscopy.
Two-dimensional gel analysis revealed the presence of six isoforms of the rLrIL-10.
The rLrIL-10 was biologically active and its administration significantly reduced serum proinflammatory cytokines, namely, interleukin 1β, TNFα, and IL-8, and augmented the NKEF transcript levels in spleen of L.
rohita.
Anti-inflammatory role of the rLrIL-10 was further established by inhibition of phagocytosis using NBT reduction assay in vitro.
The data indicate that the dimeric alpha helical structure and function of IL-10 of L.
rohita as a key regulator of anti-inflammatory response have remained conserved during evolution.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Karan, Sweta& Dash, Pujarini& Kaushik, Himani& Sahoo, Pramoda K.& Garg, Lalit C.& Dixit, Aparna. 2016. Structural and Functional Characterization of Recombinant Interleukin-10 from Indian Major Carp Labeo rohita. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108769
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Karan, Sweta…[et al.]. Structural and Functional Characterization of Recombinant Interleukin-10 from Indian Major Carp Labeo rohita. Journal of Immunology Research No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1108769
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Karan, Sweta& Dash, Pujarini& Kaushik, Himani& Sahoo, Pramoda K.& Garg, Lalit C.& Dixit, Aparna. Structural and Functional Characterization of Recombinant Interleukin-10 from Indian Major Carp Labeo rohita. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108769
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108769
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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