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Microarrays as Model Biosensor Platforms to Investigate the Structure and Affinity of Aptamers
المؤلفون المشاركون
Martin, Jennifer A.
Chushak, Yaroslav
Hagen, Joshua A.
Kelley-Loughnane, Nancy
Chávez, Jorge L.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-03
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Immobilization of nucleic acid aptamer recognition elements selected free in solution onto the surface of biosensor platforms has proven challenging.
This study investigated the binding of multiple aptamer/target pairs immobilized on a commercially available microarray as a model system mimicking biosensor applications.
The results indicate a minimum distance (linker length) from the surface and thymine nucleobase linker provides reproducible binding across varying conditions.
An indirect labeling method, where the target was labeled with a biotin followed by a brief Cy3-streptavidin incubation, provided a higher signal-to-noise ratio and over two orders of magnitude improvement in limit of detection, compared to direct Cy3-protein labeling.
We also showed that the affinities of the aptamer/target interaction can change between direct and indirect labeling and conditions to optimize for the highest fluorescence intensity will increase the sensitivity of the assay but will not change the overall affinity.
Additionally, some sequences which did not initially bind demonstrated binding when conditions were optimized.
These results, in combination with studies demonstrating enhanced binding in nonselection buffers, provided insights into the structure and affinity of aptamers critical for biosensor applications and allowed for generalizations in starting conditions for researchers wishing to investigate aptamers on a microarray surface.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Martin, Jennifer A.& Chushak, Yaroslav& Chávez, Jorge L.& Hagen, Joshua A.& Kelley-Loughnane, Nancy. 2016. Microarrays as Model Biosensor Platforms to Investigate the Structure and Affinity of Aptamers. Journal of Nucleic Acids،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108986
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Martin, Jennifer A.…[et al.]. Microarrays as Model Biosensor Platforms to Investigate the Structure and Affinity of Aptamers. Journal of Nucleic Acids No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1108986
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Martin, Jennifer A.& Chushak, Yaroslav& Chávez, Jorge L.& Hagen, Joshua A.& Kelley-Loughnane, Nancy. Microarrays as Model Biosensor Platforms to Investigate the Structure and Affinity of Aptamers. Journal of Nucleic Acids. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108986
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108986
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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