Real-Time Sensing of Hydrogen Peroxide by ITOMWCNTHorseradish Peroxidase Enzyme Electrode
المؤلفون المشاركون
Magyar, Melinda
Rinyu, László
Janovics, Róbert
Berki, Péter
Hernádi, Klára
Hajdu, Kata
Szabó, Tibor
Nagy, László
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-14
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The accurate and sensitive determination of H2O2 is very important in many cases because it is a product of reactions catalysed by several oxidase enzymes in living cells and it is essential in environmental and pharmaceutical analyses.
The fabrication of enzyme protein activity based biosensors is a very promising way for this purpose because the function of biological molecules is very specific, sensitive, and selective.
Horseradish peroxidase (HRP) is the most commonly used enzyme for H2O2 detection because it can oxidize hydrogen atoms and, for example, xenobiotics in the presence of H2O2.
In order to define the limit of detection (LOD) of H2O2 we made calibrations with guaiacol and amplex red (AR), which are hydrogen donors of HRP.
The accumulation of the reaction products, tetraguaiacol, and resorufin, respectively, then can be easily detected by absorption or emission (fluorescence) spectroscopy.
In our experiments an enzyme electrode was fabricated from ITO (indium tin oxide), functionalized multiwalled carbon nanotubes (f-MWCNTs), and HRP.
Although the enzyme activity was smaller by about two orders of magnitude when the enzyme was bound to the f-MWCNTs (ca.
10−2 M H2O2/(M HRP·sec) compared to ca.
2 M H2O2/(M HRP·sec) and 5 M H2O2/(M HRP·sec) with AR and guaiacol in buffer solution), LOD of the H2O2 decomposition was about 6 pM H2O2/sec and 10 pM H2O2/sec in the case of AR and guaiacol, respectively.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Magyar, Melinda& Rinyu, László& Janovics, Róbert& Berki, Péter& Hernádi, Klára& Hajdu, Kata…[et al.]. 2016. Real-Time Sensing of Hydrogen Peroxide by ITOMWCNTHorseradish Peroxidase Enzyme Electrode. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1109057
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Magyar, Melinda…[et al.]. Real-Time Sensing of Hydrogen Peroxide by ITOMWCNTHorseradish Peroxidase Enzyme Electrode. Journal of Nanomaterials No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1109057
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Magyar, Melinda& Rinyu, László& Janovics, Róbert& Berki, Péter& Hernádi, Klára& Hajdu, Kata…[et al.]. Real-Time Sensing of Hydrogen Peroxide by ITOMWCNTHorseradish Peroxidase Enzyme Electrode. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1109057
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1109057
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر