Development of a Loop Mediated Isothermal Amplification for Diagnosis of Ascaris lumbricoides in Fecal Samples
المؤلفون المشاركون
Shiraho, Esther A.
Eric, Agola L.
Mwangi, Ibrahim N.
Maina, Geoffrey M.
Kinuthia, Joseph M.
Mutuku, Martin W.
Mugambi, Robert M.
Mwandi, Jackson M.
Mkoji, Gerald M.
المصدر
Journal of Parasitology Research
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-11-01
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Ascaris lumbricoides is a nematode parasite that causes the common tropical infection ascariasis in humans.
It is also considered among the neglected tropical diseases.
Diagnosis relies mainly on microscopy-based methods which are laborious, are limited by low sensitivity, and require high expertise.
We have developed a loop mediated isothermal amplification (LAMP) for diagnosis of ascariasis in fecal samples, based on the first internal transcribed (ITS-1) spacer region of the ribosomal DNA.
We used Primer Explorer V4 software to design primers.
Ascaris adult and ova were obtained from naturally infected school children, whose parents/guardians gave consent for their participation in the study.
Genomic DNA was extracted using alkaline lysis method and amplified by LAMP at 63°C for 45 minutes.
LAMP products were visualized by naked eyes after adding SYBR Green dye and also on agarose gel.
LAMP successfully and reliably detected Ascaris DNA from a single egg and in fecal samples.
The assay specifically detected Ascaris DNA without amplifying DNA from ova of other parasites which commonly coexist with A.
lumbricoides in feces.
The developed LAMP assay has great potential for use in ascariasis diagnosis at the point of care and in low infection intensity situation that characterize control and elimination campaigns.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shiraho, Esther A.& Eric, Agola L.& Mwangi, Ibrahim N.& Maina, Geoffrey M.& Kinuthia, Joseph M.& Mutuku, Martin W.…[et al.]. 2016. Development of a Loop Mediated Isothermal Amplification for Diagnosis of Ascaris lumbricoides in Fecal Samples. Journal of Parasitology Research،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110244
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shiraho, Esther A.…[et al.]. Development of a Loop Mediated Isothermal Amplification for Diagnosis of Ascaris lumbricoides in Fecal Samples. Journal of Parasitology Research No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1110244
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shiraho, Esther A.& Eric, Agola L.& Mwangi, Ibrahim N.& Maina, Geoffrey M.& Kinuthia, Joseph M.& Mutuku, Martin W.…[et al.]. Development of a Loop Mediated Isothermal Amplification for Diagnosis of Ascaris lumbricoides in Fecal Samples. Journal of Parasitology Research. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110244
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1110244
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر