An EP2 Agonist Facilitates NMDA-Induced Outward Currents and Inhibits Dendritic Beading through Activation of BK Channels in Mouse Cortical Neurons
المؤلفون المشاركون
Hayashi, Yoshinori
Morinaga, Saori
Liu, Xia
Zhang, Jing
Yokoyama, Takeshi
Nakanishi, Hiroshi
Wu, Zhou
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-19
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Prostaglandin E2 (PGE2), a major metabolite of arachidonic acid produced by cyclooxygenase pathways, exerts its bioactive responses by activating four E-prostanoid receptor subtypes, EP1, EP2, EP3, and EP4.
PGE2 enables modulating N-methyl-D-aspartate (NMDA) receptor-mediated responses.
However, the effect of E-prostanoid receptor agonists on large-conductance Ca2+-activated K+ (BK) channels, which are functionally coupled with NMDA receptors, remains unclear.
Here, we showed that EP2 receptor-mediated signaling pathways increased NMDA-induced outward currents ( I NMDA-OUT ), which are associated with the BK channel activation.
Patch-clamp recordings from the acutely dissociated mouse cortical neurons revealed that an EP2 receptor agonist activated I NMDA-OUT , whereas an EP3 receptor agonist reduced it.
Agonists of EP1 or EP4 receptors showed no significant effects on I NMDA-OUT .
A direct perfusion of 3,5′-cyclic adenosine monophosphate (cAMP) through the patch pipette facilitated I NMDA-OUT , which was abolished by the presence of protein kinase A (PKA) inhibitor.
Furthermore, facilitation of I NMDA-OUT caused by an EP2 receptor agonist was significantly suppressed by PKA inhibitor.
Finally, the activation of BK channels through EP2 receptors facilitated the recovery phase of NMDA-induced dendritic beading in the primary cultured cortical neurons.
These results suggest that a direct activation of BK channels by EP2 receptor-mediated signaling pathways plays neuroprotective roles in cortical neurons.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hayashi, Yoshinori& Morinaga, Saori& Liu, Xia& Zhang, Jing& Wu, Zhou& Yokoyama, Takeshi…[et al.]. 2016. An EP2 Agonist Facilitates NMDA-Induced Outward Currents and Inhibits Dendritic Beading through Activation of BK Channels in Mouse Cortical Neurons. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111086
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hayashi, Yoshinori…[et al.]. An EP2 Agonist Facilitates NMDA-Induced Outward Currents and Inhibits Dendritic Beading through Activation of BK Channels in Mouse Cortical Neurons. Mediators of Inflammation No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1111086
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hayashi, Yoshinori& Morinaga, Saori& Liu, Xia& Zhang, Jing& Wu, Zhou& Yokoyama, Takeshi…[et al.]. An EP2 Agonist Facilitates NMDA-Induced Outward Currents and Inhibits Dendritic Beading through Activation of BK Channels in Mouse Cortical Neurons. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111086
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1111086
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر