Tumor Necrosis Factor Alpha Inhibits L-Type Ca2+ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 12 Pathway
المؤلفون المشاركون
Reyes-García, Jorge
Flores-Soto, Edgar
Solís-Chagoyán, Héctor
Sommer, Bettina
Díaz-Hernández, Verónica
García-Hernández, Luz María
Montaño, Luis M.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-30
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Tumor necrosis factor alpha (TNF-α) is a potent proinflammatory cytokine that plays a significant role in the pathogenesis of asthma by inducing hyperresponsiveness and airway remodeling.
TNF-α diminishes the L-type voltage dependent Ca2+ channel (L-VDCC) current in cardiac myocytes, an observation that seems paradoxical.
In guinea pig sensitized tracheas KCl responses were lower than in control tissues.
Serum from sensitized animals (Ser-S) induced the same phenomenon.
In tracheal myocytes from nonsensitized (NS) and sensitized (S) guinea pigs, an L-VDCC current (ICa) was observed and diminished by Ser-S.
The same decrease was detected in NS myocytes incubated with TNF-α, pointing out that this cytokine might be present in Ser-S.
We observed that a small-molecule inhibitor of TNF-α (SMI-TNF) and a TNF-α receptor 1 (TNFR1) antagonist (WP9QY) reversed ICa decrease induced by Ser-S in NS myocytes, confirming the former hypothesis.
U0126 (a blocker of ERK 1/2 kinase) also reverted the decrease in ICa.
Neither cycloheximide (a protein synthesis inhibitor) nor actinomycin D (a transcription inhibitor) showed any effect on the TNF-α-induced ICa reduction.
We found that C a V 1.2 and C a V 1.3 mRNA and proteins were expressed in tracheal myocytes and that sensitization did not modify them.
In cardiac myocytes, ERK 1/2 phosphorylates two sites of the L-VDCC, augmenting or decreasing ICa; we postulate that, in guinea pig tracheal smooth muscle, TNF-α diminishes ICa probably by phosphorylating the L-VDCC site that reduces its activity through the ERK1/2 MAP kinase pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Reyes-García, Jorge& Flores-Soto, Edgar& Solís-Chagoyán, Héctor& Sommer, Bettina& Díaz-Hernández, Verónica& García-Hernández, Luz María…[et al.]. 2016. Tumor Necrosis Factor Alpha Inhibits L-Type Ca2+ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 12 Pathway. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1111135
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Reyes-García, Jorge…[et al.]. Tumor Necrosis Factor Alpha Inhibits L-Type Ca2+ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 12 Pathway. Mediators of Inflammation No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1111135
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Reyes-García, Jorge& Flores-Soto, Edgar& Solís-Chagoyán, Héctor& Sommer, Bettina& Díaz-Hernández, Verónica& García-Hernández, Luz María…[et al.]. Tumor Necrosis Factor Alpha Inhibits L-Type Ca2+ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 12 Pathway. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1111135
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1111135
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر