Expression Profile of Cationic Amino Acid Transporters in Rats with Endotoxin-Induced Uveitis
المؤلفون المشاركون
Yang, Chang-Hao
Hsu, Yung-Ray
Chang, Shu-Wen
Lee, Yi-An
Kao, Tzu-Yun
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-16
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Purpose.
The transcellular arginine transportation via cationic amino acid transporter (CAT) is the rate-limiting step in nitric oxide (NO) synthesis, which is crucial in intraocular inflammation.
In this study, CAT isoforms and inducible nitric oxide synthase (iNOS) expression was investigated in endotoxin-induced uveitis (EIU).
Methods.
EIU was induced in Lewis rats by lipopolysaccharide (LPS) injection.
In the treatment group, the rats were injected intraperitoneally with the proteasome inhibitor bortezomib before EIU induction.
After 24 hours, leukocyte quantification, NO measurement of the aqueous humor, and histopathological examination were evaluated.
The expression of CAT isoforms and iNOS was determined by reverse transcription-polymerase chain reaction, western blotting, and immunofluorescence staining.
Nuclear factor-kappa B (NF-κB) binding activity was evaluated by electrophoretic mobility shift assay.
The mouse macrophage cell line RAW 264.7 was used to validate the in vivo findings.
Results.
LPS significantly stimulated iNOS, CAT-2A, and CAT-2B mRNA and protein expression but did not affect CAT-1 in EIU rats and RAW 264.7 cells.
Bortezomib attenuated inflammation and inhibited iNOS, CAT-2A, and CAT-2B expression through NF-κB inhibition.
Conclusions.
CAT-2 and iNOS, but not CAT-1, are specifically involved in EIU.
NF-κB is essential in the induction of CAT-2 and iNOS in EIU.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hsu, Yung-Ray& Chang, Shu-Wen& Yang, Chang-Hao& Lee, Yi-An& Kao, Tzu-Yun. 2016. Expression Profile of Cationic Amino Acid Transporters in Rats with Endotoxin-Induced Uveitis. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1111160
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hsu, Yung-Ray…[et al.]. Expression Profile of Cationic Amino Acid Transporters in Rats with Endotoxin-Induced Uveitis. Mediators of Inflammation No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1111160
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hsu, Yung-Ray& Chang, Shu-Wen& Yang, Chang-Hao& Lee, Yi-An& Kao, Tzu-Yun. Expression Profile of Cationic Amino Acid Transporters in Rats with Endotoxin-Induced Uveitis. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1111160
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1111160
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر