Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3KAkt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis
المؤلفون المشاركون
Wang, Wei
Zhou, Pang-Hu
Hu, Wei
Zhang, Xiao-Bin
Zhang, Li-Jun
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-04-26
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
This study was carried out to investigate whether ADM can modulate LPS-induced inflammation and apoptosis in rat Leydig cells.
Leydig cells were treated with ADM before LPS-induced cytotoxicity.
We determined the concentrations of ROS, MDA, GSH, LDH, and testosterone and the MMP.
The mRNA levels of IL-1, IL-6, iNOS, and COX-2 were obtained, and the concentrations of IL-1, IL-6, NO, and PGE2 were determined.
Apoptosis was assessed by TUNEL and detection of DNA fragmentation.
The levels of mRNA and protein were determined for Bcl-2, Bax, caspase-3, and PARP.
The protein contents for total and p-Akt were measured.
ADM pretreatment significantly elevated the MMP and testosterone concentration and reduced the levels of ROS, MDA, GSH, and LDH.
ADM pretreatment significantly decreased the mRNA levels of IL-1, IL-6, iNOS, and COX-2 and the concentrations of IL-1, IL-6, NO, and PGE2.
LPS-induced TUNEL-positive Leydig cells were significantly decreased by ADM pretreatment, a result further confirmed by decreased DNA fragmentation.
ADM pretreatment decreased apoptosis by significantly promoting Bcl-2 and inhibiting Bax, caspase-3, and PARP expressions.
The LPS activity that reduced p-Akt level was significantly inhibited by ADM pretreatment.
ADM protected rat Leydig cells from LPS-induced inflammation and apoptosis, which might be associated with PI3K/Akt mitochondrial signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Pang-Hu& Hu, Wei& Zhang, Xiao-Bin& Wang, Wei& Zhang, Li-Jun. 2016. Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3KAkt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1111201
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Pang-Hu…[et al.]. Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3KAkt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis. Mediators of Inflammation No. 2016 (2016), pp.1-16.
https://search.emarefa.net/detail/BIM-1111201
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Pang-Hu& Hu, Wei& Zhang, Xiao-Bin& Wang, Wei& Zhang, Li-Jun. Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3KAkt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1111201
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1111201
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر