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Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats
المؤلفون المشاركون
Munoz, Pablo
Estay, Carolina
Díaz, Paula
Elgueta, Claudio
Ardiles, Álvaro O.
Lizana, Pablo A.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-07-14
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Although the importance of DNA methylation-dependent gene expression to neuronal plasticity is well established, the dynamics of methylation and demethylation during the induction and expression of synaptic plasticity have not been explored.
Here, we combined electrophysiological, pharmacological, molecular, and immunohistochemical approaches to examine the contribution of DNA methylation and the phosphorylation of Methyl-CpG-binding protein 2 (MeCP2) to synaptic plasticity.
We found that, at twenty minutes after theta burst stimulation (TBS), the DNA methylation inhibitor 5-aza-2-deoxycytidine (5AZA) impaired hippocampal long-term potentiation (LTP).
Surprisingly, after two hours of TBS, when LTP had become a transcription-dependent process, 5AZA treatment had no effect.
By comparing these results to those in naive slices, we found that, at two hours after TBS, an intergenic region of the RLN gene was hypomethylated and that the phosphorylation of residue S80 of MeCP2 was decreased, while the phosphorylation of residue S421 was increased.
As expected, 5AZA affected only the methylation of the RLN gene and exerted no effect on MeCP2 phosphorylation patterns.
In summary, our data suggest that tetanic stimulation induces critical changes in synaptic plasticity that affects both DNA methylation and the phosphorylation of MeCP2.
These data also suggest that early alterations in DNA methylation are sufficient to impair the full expression of LTP.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Munoz, Pablo& Estay, Carolina& Díaz, Paula& Elgueta, Claudio& Ardiles, Álvaro O.& Lizana, Pablo A.. 2016. Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats. Neural Plasticity،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1113157
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Munoz, Pablo…[et al.]. Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats. Neural Plasticity No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1113157
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Munoz, Pablo& Estay, Carolina& Díaz, Paula& Elgueta, Claudio& Ardiles, Álvaro O.& Lizana, Pablo A.. Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats. Neural Plasticity. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1113157
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1113157
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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