ATP Induces Disruption of Tight Junction Proteins via IL-1 Beta-Dependent MMP-9 Activation of Human Blood-Brain Barrier In Vitro
المؤلفون المشاركون
Yang, Fuxing
Zhao, Kai
Zhang, Xiufeng
Zhang, Jun
Xu, Bainan
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-04
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Disruption of blood-brain barrier (BBB) follows brain trauma or central nervous system (CNS) stress.
However, the mechanisms leading to this process or the underlying neural plasticity are not clearly known.
We hypothesized that ATP/P2X7R signaling regulates the integrity of BBB.
Activation of P2X7 receptor (P2X7R) by ATP induces the release of interleukin-1β (IL-1β), which in turn enhances the activity of matrix metalloproteinase-9 (MMP-9).
Degradation of tight junction proteins (TJPs) such as ZO-1 and occludin occurs, which finally contributes to disruption of BBB.
A contact coculture system using human astrocytes and hCMEC/D3, an immortalized human brain endothelial cell line, was used to mimic BBB in vitro.
Permeability was used to evaluate changes in the integrity of TJPs.
ELISA, Western blot, and immunofluorescent staining procedures were used.
Our data demonstrated that exposure to the photoreactive ATP analog, 3′-O-(4-benzoyl)benzoyl adenosine 5′-triphosphate (BzATP), induced a significant decrease in ZO-1 and occludin expression.
Meanwhile, the decrease of ZO-1 and occludin was significantly attenuated by P2X7R inhibitors, as well as IL-1R and MMP antagonists.
Further, the induction of IL-1β and MMP-9 was closely linked to ATP/P2X7R-associated BBB leakage.
In conclusion, our study explored the mechanism of ATP/P2X7R signaling in the disruption of BBB following brain trauma/stress injury, especially focusing on the relationship with IL-1β and MMP-9.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Fuxing& Zhao, Kai& Zhang, Xiufeng& Zhang, Jun& Xu, Bainan. 2016. ATP Induces Disruption of Tight Junction Proteins via IL-1 Beta-Dependent MMP-9 Activation of Human Blood-Brain Barrier In Vitro. Neural Plasticity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113366
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Fuxing…[et al.]. ATP Induces Disruption of Tight Junction Proteins via IL-1 Beta-Dependent MMP-9 Activation of Human Blood-Brain Barrier In Vitro. Neural Plasticity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1113366
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Fuxing& Zhao, Kai& Zhang, Xiufeng& Zhang, Jun& Xu, Bainan. ATP Induces Disruption of Tight Junction Proteins via IL-1 Beta-Dependent MMP-9 Activation of Human Blood-Brain Barrier In Vitro. Neural Plasticity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113366
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1113366
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر