Partial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF-1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue
المؤلفون المشاركون
Zhang, Lirong
Hu, Yu
Xi, Ning
Song, Jie
Huang, Wenjing
Song, Shanshan
Liu, Yiting
Liu, Xianying
Xie, Yingjun
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-11-15
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Hypoxia is prognostically important in colorectal cancer (CRC) therapy.
Partial oxygen pressure (pO2) is an important parameter of hypoxia.
The correlation between pO2 levels and expression levels of prognostic biomarkers was measured in CRC tissues.
Human CRC tissues were collected and pO2 levels were measured by OxyLite.
Three methods for tissue fixation were compared, including formalin, Finefix, and Finefix-plus-microwave.
Immunohistochemistry (IHC) staining was conducted by using the avidin-biotin complex technique for detecting the antibodies to hypoxia inducible factor-1 (HIF-1) alpha, cytokeratin 20 (CK20), and cell proliferation factor Ki67.
The levels of pO2 were negatively associated with the size of CRC tissues.
Finefix-plus-microwave fixation has the potential to replace formalin.
Additionally, microwave treatment improved Finefix performance in tissue fixation and protein preservation.
The percentage of positive cells and gray values of HIF-1 alpha, CK20, and Ki67 were associated with CRC development (P<0.05).
The levels of pO2 were positively related with the gray values of Ki67 and negatively related with the values of HIF-1 alpha and CK20 (P<0.05).
Thus, the levels of microenvironmental pO2 affect the expression of predictive biomarkers HIF-1 alpha, CK20, and Ki67 in the development of CRC tissues.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Lirong& Hu, Yu& Xi, Ning& Song, Jie& Huang, Wenjing& Song, Shanshan…[et al.]. 2016. Partial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF-1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113598
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Lirong…[et al.]. Partial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF-1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1113598
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Lirong& Hu, Yu& Xi, Ning& Song, Jie& Huang, Wenjing& Song, Shanshan…[et al.]. Partial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF-1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113598
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1113598
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر