On the Anticataractogenic Effects of L-Carnosine: Is It Best Described as an Antioxidant, Metal-Chelating Agent or Glycation Inhibitor?

المؤلفون المشاركون

Pierscionek, Barbara K.
Abdelkader, Hamdy
Longman, Michael
Alany, Raid G.

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-10-16

دولة النشر

مصر

عدد الصفحات

11

التخصصات الرئيسية

الأحياء

الملخص EN

Purpose.

L-Carnosine is a naturally occurring dipeptide which recently gained popularity as an anticataractogenic agent due to its purported antioxidant activities.

There is a paucity of research and conclusive evidence to support such claims.

This work offers compelling data that help clarify the mechanism(s) behind the anticataract properties of L-carnosine.

Methods.

Direct in vitro antioxidant free radical scavenging properties were assayed using three different antioxidant (TEAC, CUPRAC, and DPPH) assays.

Indirect in vitro and ex vivo antioxidant assays were studied by measuring glutathione bleaching capacity and total sulfhydryl (SH) capacity of bovine lens homogenates as well as hydrogen-peroxide-stress assay using human lens epithelial cells.

Whole porcine lenses were incubated in high galactose media to study the anticataract effects of L-carnosine.

MTT cytotoxicity assays were conducted on human lens epithelial cells.

Results.

The results showed that L-carnosine is a highly potent antiglycating agent but with weak metal chelating and antioxidant properties.

There were no significant decreases in lens epithelial cell viability compared to negative controls.

Whole porcine lenses incubated in high galactose media and treated with 20 mM L-carnosine showed a dramatic inhibition of advanced glycation end product formation as evidenced by NBT and boronate affinity chromatography assays.

Conclusion.

L-Carnosine offers prospects for investigating new methods of treatment for diabetic cataract and any diseases that are caused by glycation.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Abdelkader, Hamdy& Longman, Michael& Alany, Raid G.& Pierscionek, Barbara K.. 2016. On the Anticataractogenic Effects of L-Carnosine: Is It Best Described as an Antioxidant, Metal-Chelating Agent or Glycation Inhibitor?. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113795

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Abdelkader, Hamdy…[et al.]. On the Anticataractogenic Effects of L-Carnosine: Is It Best Described as an Antioxidant, Metal-Chelating Agent or Glycation Inhibitor?. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113795

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Abdelkader, Hamdy& Longman, Michael& Alany, Raid G.& Pierscionek, Barbara K.. On the Anticataractogenic Effects of L-Carnosine: Is It Best Described as an Antioxidant, Metal-Chelating Agent or Glycation Inhibitor?. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113795

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1113795