Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation
المؤلفون المشاركون
Grzesiak, Jakub
Śmieszek, Agnieszka
Kornicka, Katarzyna
Szłapka, Jolanta
Marycz, Krzysztof
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-08
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
Equine metabolic syndrome (EMS) is mainly characterized by insulin resistance, obesity, and local or systemic inflammation.
That unfriendly environment of adipose tissue has huge impact on stem cells population (ASC) residing within.
In the present study, using molecular biology techniques and multiple imaging techniques (SEM, FIB-SEM, and confocal microscopy), we evaluated the impact of EMS on ASC viability and chondrogenic differentiation.
Moreover, we visualized the mitochondrial network and dynamics in ASCCTRL and ASCEMS during control and chondrogenic conditions.
In control conditions, ASCEMS were characterized by increased mitochondrial fission in comparison to ASCCTRL.
We found that extensive remodeling of mitochondrial network including fusion and fission occurs during early step of differentiation.
Moreover, we observed mitochondria morphology deterioration in ASCEMS.
These conditions seem to cause autophagic shift in ASCEMS, as we observed increased accumulation of LAMP2 and formation of multiple autophagosomes in those cells, some of which contained dysfunctional mitochondria.
“Autophagic” switch may be a rescue mechanism allowing ASCEMS to clear impaired by ROS proteins and mitochondria.
Moreover it provides a precursors-to-macromolecules synthesis, especially during chondrogenesis.
Our data indicates that autophagy in ASCEMS would be crucial for the quality control mechanisms and maintenance of cellular homeostasis ASCEMS allowing them to be in “stemness” status.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Marycz, Krzysztof& Kornicka, Katarzyna& Grzesiak, Jakub& Śmieszek, Agnieszka& Szłapka, Jolanta. 2016. Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1113851
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Marycz, Krzysztof…[et al.]. Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-18.
https://search.emarefa.net/detail/BIM-1113851
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Marycz, Krzysztof& Kornicka, Katarzyna& Grzesiak, Jakub& Śmieszek, Agnieszka& Szłapka, Jolanta. Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1113851
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1113851
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر